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Rapid multi-pass high-sensitivity ultramicro microlens imaging immunoassay instrument

An immunoassay and microlens technology, applied in the field of optics, can solve the problem that the antigen and antibody detection technology cannot be satisfied or roughly satisfied at the same time, and achieve the effect of simple and fast detection process, accurate measurement, and simple and light equipment

Active Publication Date: 2017-04-19
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, none of the existing antigen and antibody detection technologies can meet or roughly meet the above six requirements at the same time.

Method used

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  • Rapid multi-pass high-sensitivity ultramicro microlens imaging immunoassay instrument

Examples

Experimental program
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Embodiment 1

[0047] The parallel light source irradiation system of the embodiment of the fast multi-pass high-sensitivity ultra-micro lens imaging immunoassay instrument of the present invention, such as figure 1 As shown, it adopts the Pomeas LTD type parallel light source, and the divergence angle of the parallel light source is less than 0.2 degrees. The parallel light source irradiation system uses LED lamp beads with a power of 5W and a wavelength of 532nm as the light source, and finally forms a circular spot with a diameter of 5cm. And does not change with the distance.

[0048] The high-resolution automatic optical focusing imaging system in the embodiment of the present invention, such as figure 2 As shown, it is composed of a microscopic objective lens, a high-definition CCD camera, a stepper motor drive system to control the focus of the microscopic objective lens, and an image recognition analysis and control software, so as to realize the automatic search and recognition of ...

Embodiment 2

[0057] Different from Example 1 of the present invention, a 16-channel microlens imaging immunoassay instrument is designed in Example 2. In Example 2, the fast multi-pass high-sensitivity ultra-micro-lens imaging immunoassay instrument of the present invention adopts the invention patent application (201610423773.X) "a micro-lens (or micro-lens array) imaging detection plate" disclosed The 16-channel porous microlens imaging detection plate can realize 16-channel real-time rapid detection of trace antigens or antibodies. The used objective lens of imaging is Computar company 8mm fixed-focus lens among the embodiment 2, and camera adopts Olympus DP73 type CCD camera, and pixel is 17,300,000, and display screen is Camry's 12.1 inch capacitive high-definition touch screen, and other part structures are identical with embodiment 1. The length, width and height of the instrument are 28cm, 23cm and 26cm respectively, and the weight is about 5kg.

[0058] The 16-channel immunoassay c...

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Abstract

The invention discloses a rapid multi-pass high-sensitivity ultramicro microlens imaging immunoassay instrument, which comprises a parallel light source irradiation system, a high-resolution automatic optical focusing imaging system, a sample cell high-precision temperature-control system, a microcomputer, and an intelligent autoanalysis software and display system. Through automatic rapid focusing imaging of a microlens immersed in an antigen and antibody solution and analysis of imaging parameters, refractive index change of the solution before and after the reaction of the antigen and antibody is measured at real time, so as to further quantitatively determine concentration of the antigen or antibody and display relevant results. The microlens imaging immunoassay instrument has a small structure and is portable for field use. The whole detection process is intelligent, automatic, simple and convenient without the need of coating beforehand and washing afterwards; and there is no need to add enzyme-labelled substance, chromogenic substrate and auxiliary kits or chromatographic strips, etc. Only several microliters of a sample are needed; multi-pass sample detection can be completed within several minutes; and the measuring sensitivity reaches -pg / mL order of magnitude.

Description

technical field [0001] The invention belongs to the field of optical technology, and relates to an optical antigen-antibody detection instrument, in particular to a rapid multi-pass high-sensitivity ultra-micro-lens imaging immunoassay instrument for detecting the concentration of antigen or antibody in a solution by measuring the change of the refractive index of the solution. Background technique [0002] Immunoassay is one of the most important and frequently used assays in biology, basic medical research and clinical research, food safety, and environmental monitoring. Therefore, the demand and requirements for the detection of antigen-antibody reactions are getting higher and higher. At present, the development direction and requirements of antigen-antibody reaction detection technology mainly include six aspects. The first is to achieve fast, simple and multi-pass detection, especially without any cumbersome operations such as labeling, prior modification, post-washing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/41
CPCG01N21/41G01N21/77G01N2021/0346G01N2021/7776G01N2201/0446G01N33/5302G01N21/6486G01N21/76G01N2021/6463G01N21/6456G01J1/0403G01N21/6452
Inventor 黄耀熊何江
Owner JINAN UNIVERSITY
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