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Expansion of lymphocytes with a cytokine composition for active cellular immunotherapy

A technology of lymphocytes and cytokines, applied in the field of cytokine composition and clinically relevant lymphocytes

Active Publication Date: 2017-04-19
保利比奥斯博特有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only about 15 to 20% of patients have resectable tumors, so the disease has the potential to be cured

Method used

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  • Expansion of lymphocytes with a cytokine composition for active cellular immunotherapy
  • Expansion of lymphocytes with a cytokine composition for active cellular immunotherapy
  • Expansion of lymphocytes with a cytokine composition for active cellular immunotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0215] Example 1 - Lymphocyte Expansion Protocol Using Peripheral Blood Mononuclear Cells

[0216] A) Materials and Instruments

[0217] i) Instrument

[0218] 24-well plate, (Becton, Dickinson (BD), REF 353504)

[0219] sterile scalpel

[0220] sterile tweezers

[0221] Medimachine (Tissue Homogenizer), (BD, catalog number: 340588 )

[0222] Medicon, 50 μm, (BD, catalog number: 340591 )

[0223] Filcons, 200 μm (BD, catalog number: 340613 )

[0224] Laminar Flow Hood (Level 2 Biological Safety Hood)

[0225] Low temperature freezer, –80°C

[0226] refrigerator

[0227] centrifuge

[0228] ii) Supplies

[0229] gloves (latex)

[0230] lab coat

[0231] Sterile centrifuge tube, 15mL.

[0232] Pipet tips

[0233] Pipette aid

[0234] waste container

[0235] iii) Reagents

[0236] RPMI 1640, (Gibco, REF 61870-044)

[0237] Cellgro, (CellGenix, Cat: 20801-0100)

[0238] Mixed human AB serum (Innoative Research, IPLA-SerAB-13458)

[0239] Fetal bovine ser...

Embodiment 2

[0248] Example 2 Lymphocyte Expansion Protocol Using Peripheral Blood Mononuclear Cells

[0249] The procedure of Example 2 is the same as that of Example 1, except that the donor is a patient receiving NY-ESO-1 vaccination.

Embodiment 3

[0250] Example 3 - Lymphocyte Expansion Protocol Using Peripheral Blood Mononuclear Cells

[0251] Example 3 is the same as Example 1, except that the donor is a patient whose tumor already expresses TAA NY-ESO-1.

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Abstract

The present invention relates to a composition for expanding lymphocytes comprising at least two types of cytokines selected from interleukin 2 (IL-2), interleukin 15 (IL-15) and interleukin 21 (IL-21). It further relates to a Method of preparing a population of clinically relevant lymphocytes, comprising the steps of: obtaining a body sample from a mammal in particular a tissue sample or body liquid sample, comprising at least one lymphocyte and optionally separating the cells in the body sample, culturing the body sample in-vitroto expand and / or stimulate lymphocytes in the sample wherein the culturing comprises using IL-2, IL-15 and / or IL-21, and optionally determining the presence of clinically relevant lymphocyte in the cultured sample.The present invention also relates to an immunotherapy and the population of clinically relevant lymphocytes.

Description

technical field [0001] The present invention relates to active cellular immunotherapy comprising methods of producing clinically relevant lymphocyte populations using predetermined cytokine compositions. The invention also relates to cytokine compositions and the resulting clinically relevant lymphocytes. Background technique [0002] Cancer remains one of the most common causes of death in developed countries. For example, it is the second most common cause of death in the United States and Germany, with 560,000 (2009) and 218,000 (2010) deaths, respectively. Despite improvements in the ability to detect and treat this range of diseases, survival rates for many cancers remain low. [0003] Among cancer diseases, pancreatic cancer is the fourth leading cause of cancer death in the United States and Sweden, and shows no sign of improvement. By the time pancreatic cancer is diagnosed, most patients with locally advanced cancer or metastatic disease are not cured and are per...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783A61K35/17A61P31/00A61P35/00A61P37/02
CPCA61K35/17C12N5/0636C12N2501/2315C12N2501/2302C12N2501/2321C12N2501/515A61K39/0011A61K38/20A61K38/21A61K35/14A61K35/26G01N33/5047G01N2333/54G01N2333/55A61P35/00A61P37/00A61P31/00A61K38/19G01N33/50A61K2035/124
Inventor 马库斯·莫伊雷尔
Owner 保利比奥斯博特有限责任公司
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