Method for increasing content of mogroside IV

A technology of mogroside and Luo Han Guo, applied in horticultural methods, botanical equipment and methods, gardening, etc., can solve the problems of restricting the healthy development of the Luo Han Guo industry and low content, achieve strong practicability and promotion value, enrich the growth environment, improve The effect on survival

Inactive Publication Date: 2017-04-26
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that glucoside V is transformed from low glycosides such as glycoside IV, especially at the later stage of fruit development, glucoside IV and other low glycosides are completely consumed and converted into sweet glycoside V, however, glucosid...

Method used

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  • Method for increasing content of mogroside IV
  • Method for increasing content of mogroside IV
  • Method for increasing content of mogroside IV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for increasing the content of mogroside IV, comprising the following steps:

[0036] Step 1, preparing a solid medium containing methyl jasmonate, the concentration of methyl jasmonate before the solidification of the solid medium is 220 μmol / L;

[0037] Step 2: inoculate the Luo Han Guo tissue-cultured seedlings in a solid medium, and transplant them to the field after culturing for 30 days.

[0038] Applying methyl jasmonate to the mogroside tissue culture seedlings can induce the high expression of the key enzymes of mogroside IV metabolism (such as glucosyltransferase and cytochrome P450 enzymes, etc.), thereby rapidly increasing the content of mogroside IV in a short period of time.

[0039]In the method for increasing the content of mogroside IV, the culture conditions for inoculating the mogroside plantlets in the solid medium in the step 2 are as follows: the relative humidity is 66%, the light intensity is 1400 lux, and the light time is 8h / d and a te...

Embodiment 2

[0046] On the basis of Example 1, in the method for increasing the content of mogroside IV, after the second step, it also includes:

[0047] Step 3, dissolving methyl jasmonate in ethanol, and preparing a methyl jasmonate solution with a concentration of 200 μmol / L, spraying the methyl jasmonate solution once every morning, noon and evening every day 55 days after the pollination of Luo Han Guo, until the leaves Spray continuously for 10 days until the surface drips. The next day, samples were taken, and the content of mogroside IV was detected by HPLC.

[0048] Methyl jasmonate was applied when planting Luo Han Guo to induce the high expression of key enzymes in the metabolism of mogroside IV (such as glucosyltransferase and cytochrome P450 enzymes, etc.), thereby rapidly increasing the content of mogroside IV in a short period of time.

Embodiment 3

[0050] On the basis of Example 2, such as figure 1 As shown, in the method for increasing the content of mogroside IV, the specific steps of transplanting the mogroside plantlets to the field in the second step are:

[0051] Dig a planting pit with a depth of 11cm in the field, and lay a polyvinyl alcohol film on the bottom of the planting pit to prevent the sterilized seedling substrate from contacting the soil at the bottom of the planting pit, and prevent the occurrence of soil-borne diseases. The polyvinyl alcohol film is degradable, It will not pollute the environment, nor will it hinder the downward growth of the roots of the seedlings. Then insert the seedling cup in the planting pit, and then pour 150 of the sterilized seedling substrate into the seedling cup to the same height as the soil layer outside the planting pit. Provide a sterile growth environment, the seedling substrate is any kind of substrate used in the cultivation of Luo Han Guo, but it has been steriliz...

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Abstract

The invention discloses a method for increasing the content of mogroside IV. The method comprises the following steps: I, preparing a solid culture medium containing jasmonic acid methyl ester, wherein the concentration of the jasmonic acid methyl ester is 50-400 [mu] mol/L before the solid culture medium is cured; and II, inoculating momordica grosvenoritissue culture seedlings into the solid culture medium, culturing for 28-35 days, and transplanting the momordica grosvenori culture seedlings into fields. The method has the characteristics of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or reside in products, and the like, and by adopting the method, the yield of the mogroside IV can be effectively increased, and large-scale production of the mogroside IV can be achieved.

Description

technical field [0001] The invention relates to the technical field of mangosteen cultivation. More specifically, the present invention relates to a method for increasing the content of mogroside IV. Background technique [0002] Luo Han Guo is a precious medicinal and sweet plant unique to China. Its fruit is cool in nature and sweet in taste, and has the functions of clearing away heat and moistening lungs, relieving throat and opening sound, laxative and anticancer. The active ingredient glucoside V is one of the strongest non-sugar sweeteners in the world. It is 300-400 times higher than sugar and is widely used in food, health products and medicines. It is an ideal sugar substitute for diabetics, obese people and hypertensive patients. Studies have found that glucoside V is transformed from low glycosides such as glycoside IV, especially in the later stage of fruit development, glucoside IV and other low glycosides are completely consumed and converted into sweet glyc...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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