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Preparation method of a three-dimensional structure polyglycerol sebacate-based material macroporous scaffold

A polyglycerol sebacate, three-dimensional structure technology, applied in the direction of prosthesis, medical science, etc., can solve the problems of inconvenient preparation, uneven salt density, uneven crystal density, etc., and is beneficial to environmental protection, The effect of reducing negative effects and simple operation steps

Inactive Publication Date: 2019-06-04
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) In a mold with a complex three-dimensional structure, if the cavity is too large, the crystallization density will be uneven, and it will be difficult to make the salt contact with each other through crystallization, so it is difficult to prepare a complex three-dimensional structure scaffold;
[0005] (2) Salt crystals are prone to collapse during the process of pouring the PGS-based material solution, which is inconvenient to prepare
[0007] (1) Although the salt grains used have been ground, the grain size cannot be guaranteed to be uniform;
[0008] (2) In the complex three-dimensional mold with filling pressure, the density of salt in each part is uneven due to uneven force

Method used

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  • Preparation method of a three-dimensional structure polyglycerol sebacate-based material macroporous scaffold
  • Preparation method of a three-dimensional structure polyglycerol sebacate-based material macroporous scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Dissolving gelatin in water to form a 3% (w / v) aqueous solution is poured into a three-dimensional mold with a certain three-dimensional structure, frozen at -20°C for 1 day, and placed in a freeze dryer to freeze-dry to obtain gelatin large The pore scaffold acts as a porogen;

[0037] (2) Dissolving PGSLP in tetrahydrofuran to prepare a 10% (w / v) PGS-based material solution;

[0038] (3) Soak the gelatin macroporous scaffold in step (1) in the PGS-based material solution in step (2), place it in a vacuum drying oven, and evacuate it circularly at a vacuum degree of less than 10 Pa until no bubbles are generated , indicating that the solution is completely immersed in the macropores of the gelatin support, and then the gelatin support that absorbs the PGS-based material solution is taken out and placed in a vacuum oven at a temperature of 150°C and an air pressure of 100Pa for 48 hours to evaporate the solvent and solidify the PGS-based material ;

[0039] (4) So...

Embodiment 2

[0041] (1) Dissolving gelatin in water to form a 1% (w / v) aqueous solution is poured into a three-dimensional mold with a certain three-dimensional structure, frozen at -20°C for 1 day, and placed in a freeze dryer to freeze-dry to obtain gelatin large The pore scaffold acts as a porogen;

[0042] (2) Dissolving PGSLP in absolute ethanol to prepare a 10% (w / v) PGS-based material solution;

[0043] (3) Soak the gelatin macroporous scaffold in step (1) in the PGS-based material solution in step (2), place it in a vacuum drying oven, and evacuate it circularly at a vacuum degree of less than 10 Pa until no bubbles are generated , indicating that the solution is completely immersed in the macropores of the gelatin support, and then the gelatin support that absorbs the PGS-based material solution is taken out and placed in a vacuum oven at a temperature of 150°C and an air pressure of 100Pa for 48 hours to evaporate the solvent and solidify the PGS-based material ;

[0044] (4) S...

Embodiment 3

[0046] (1) Dissolve gelatin in water and prepare a 5% (w / v) aqueous solution, pour it into a three-dimensional mold with a certain three-dimensional structure, freeze at -20°C for 1 day, and freeze-dry in a freeze dryer to obtain gelatin large The pore scaffold acts as a porogen;

[0047] (2) dissolving polyglycerol sebacate (PGS) in 1,4-dioxane to prepare a 10% (w / v) PGS-based material solution;

[0048] (3) Soak the gelatin macroporous scaffold in step (1) in the PGS-based material solution in step (2), place it in a vacuum drying oven, and evacuate it circularly at a vacuum degree of less than 10 Pa until no bubbles are generated , indicating that the solution is completely immersed in the macropores of the gelatin support, and then the gelatin support that absorbs the PGS-based material solution is taken out, and placed in a vacuum oven at a temperature of 150°C and an air pressure of 100Pa for 96 hours to volatilize the solvent and solidify the PGS-based material ;

[0...

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Abstract

The invention relates to a production method of a three-dimensional structured poly(glycerol-sebacate)-based macro-porous scaffold. The production method comprises the following steps: pouring an aqueous gelatin solution to a three-dimensional die, and refrigerating the die in a -20 - -80 DEG C for 4-24 h to obtain a three-dimensional structured gelatin macro-porous scaffold; dissolving a poly(glycerol-sebacate) (PGS) base material in a solvent to obtain a PGS base material solution; and immersing the gelatin macro-porous scaffold in the PGS base material solution under a vacuum condition to make the solution completely enter gaps of the gelatin macro-porous scaffold in order to obtain a PGS base material solution adsorbed gelatin scaffold, taking out the scaffold, solidifying the scaffold, cleaning the solidified scaffold, and freeze-drying the cleaned scaffold in order to obtain the three-dimensional structured poly(glycerol-sebacate)-based macro-porous scaffold. Compared with traditional macro-porous scaffold production methods, the method disclosed in the invention has the advantages of simplicity in operation and low production cost.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a preparation method of a three-dimensional polyglycerol sebacate-based material macroporous scaffold. Background technique [0002] Polyglycerol sebacate (PGS)-based material is a kind of thermosetting material. Its prepolymer is viscous at room temperature and cannot be directly applied to the preparation of scaffolds. It needs to be heated under high temperature conditions without oxygen and low pressure. Curing can be used for tissue engineering scaffold applications, and the cured PGS-based material has good biocompatibility, biodegradability and elastic properties. The preparation of PGS-based materials into macroporous scaffolds with complex three-dimensional structures is currently a difficult research point, because conventional porogenic techniques, such as gas foaming, freeze-drying, etc., cannot be directly combined with the thermal curing process of PGS-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/22
CPCA61L27/18A61L27/222C08L89/00C08L67/00
Inventor 何创龙杜海波王伟忠
Owner DONGHUA UNIV
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