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Aspergillus niger NJDL-12 bacterial strain and application thereof to improvement of coastal saline-alkali soil

A technology of NJDL-12 and Aspergillus niger, applied in the field of environmental biology, can solve the problems of improper disposal of livestock and poultry manure, pollute the environment, waste nutrient resources, etc., achieve the effect of increasing total phosphorus content, increasing soil fertility, and promoting crop growth

Inactive Publication Date: 2017-04-26
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] On the other hand, incineration of crop stalks and improper disposal of livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste nutrient resources

Method used

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  • Aspergillus niger NJDL-12 bacterial strain and application thereof to improvement of coastal saline-alkali soil
  • Aspergillus niger NJDL-12 bacterial strain and application thereof to improvement of coastal saline-alkali soil
  • Aspergillus niger NJDL-12 bacterial strain and application thereof to improvement of coastal saline-alkali soil

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Experimental program
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Embodiment Construction

[0031] 1. Isolation and identification of strains

[0032] Soybean rhizosphere soil was collected, and in Ca 3 (PO 4 ) 2 To isolate phosphorus-dissolving fungi in the selective culture of the only phosphorus source, the size of the phosphorus-dissolving circle is used as the primary screening index, and then the liquid shake flask experiment is used to detect the dissolved Ca of the strain. 3 (PO 4 ) 2 and the ability of hydroxyapatite, the strains with high organic acid production were obtained through preliminary screening, and then multi-functional strains were screened through salt tolerance and acid-base tolerance experiments, and further verified by pot experiments in saline-alkali soil. It was found that strain NJDL-12 was using Ca 3 (PO 4 ) 2 In the culture medium as the sole phosphorus source, the soluble phosphorus concentration reached 1350mg / L after 8 days of cultivation, and in the medium with hydroxyapatite as the only phosphorus source, the soluble phosph...

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Abstract

The invention belongs to intensive agricultural production techniques and discloses an aspergillus niger NJDL-12 bacterial strain and application thereof to improvement of coastal saline-alkali soil. The bacterial strain NJDL-12 obtained by separation belongs to aspergillus niger and is preserved in the China General Microbiological Culture Collection Center on October 26, 2015, and a preservation number of the strain is CGMCC NO.11544. The aspergillus niger NJDL-12 is capable of secreting a large quantity of oxalic acid which is combined with Ca<2+> in the saline-alkali soil to form calcium oxalate, so that Ca<2+> in the soil can be consolidated to reduce soil salinity, soil aggregate structure can be improved, and soil porosity is improved. After a biological organic fertilizer prepared from the strain NJDL-12 is applied to soil, soil pH and salinity are declined, and soil aggregate structure improvement, saline-alkali soil improvement, crop growth promotion and remarkable application effects are realized.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and relates to an Aspergillus niger NJDL-12 strain and its application in improvement of coastal saline-alkali soil. The Aspergillus niger NJDL-12 strain is used to produce bio-organic fertilizer, increase the biological activity of coastal saline-alkali soil, and improve salt Alkaline soil aggregate structure, and fertilize the soil, improve and promote crop growth. Background technique [0002] With the rapid development of my country's economy, the problem of shortage of land resources is becoming more and more serious, and the reclamation of waste land has become the key direction of the development and utilization of my country's land resources. my country's coastal areas have a large area of ​​saline-alkali wasteland. Although saline-alkali soil is an important land resource, it has a harsh ecological environment such as high salinity, small soil porosity, easy compaction, poor ai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C09K17/14C12R1/685C09K101/00C09K109/00
CPCC05C11/00C05F11/08C09K17/14C09K2101/00C09K2109/00C12N1/14C12N1/145C12R2001/685C05F3/00
Inventor 王世梅黄启为沈其荣李真陈莎莎冯哲叶邓照亮杨华沈标
Owner NANJING AGRICULTURAL UNIVERSITY
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