Method of producing fermented soybean meal by using multi-strain mixed solid secondary fermentation technology and application thereof
A technology for secondary fermentation and fermentation of soybean meal, applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of single fermentation function, etc., to solve the problem of long fermentation time, increase solubility, reduce diarrhea rate and The effect of death rate
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[0035] The preparation method of each strain and mixed strain fermented liquid is as follows:
[0036] Inoculate the four original strains: strain 1 N1, strain 2 BS2, strain 3 Y3, and strain 4 AN4 into their respective appropriate medium to produce primary seed liquid under appropriate conditions, and transfer to the second strain in the logarithmic growth phase. The first-grade seed liquid fermentation tank is expanded to cultivate, the second-grade seed fermentation liquid is cultivated until the growth of the strain is in the logarithmic growth phase, and then transferred to the production tank for fermentation. When the logarithmic growth phase is reached, the fermentation is stopped, and the separately fermented bacterial liquid is used as needed After mixing, it is the mixed strain fermented liquid suitable for the production of fermented soybean meal.
Embodiment 1
[0038] Primary fermentation: Take 1000g of soybean meal (42.0% protein content), add 100g of bran, adjust the pH to 5.0-6.0, and adjust the moisture content of the fermented material to 60%. After sterilizing, cooling, and inoculating with strains, the amount of mixed strains fermented liquid added is 3% of the mass of defatted soybean meal, the fermentation temperature is 31°C, and the fermentation time is 102 hours. The acid-soluble protein content in fermented soybean meal is determined as 49.6%.
[0039] Fermentation strains: strain 1 N1, strain 2 BS2, strain mixing ratio N1: BS2 = 2: 1.
[0040] Secondary fermentation: Take 1000g of the intermediate product after primary fermentation, adjust the pH to 5.0-6.0, and adjust the moisture content of the fermentation material to 60%. Sterilized, cooled, inoculated with strains, the amount of mixed strains fermented liquid (mixed ratio of strains N1: BS2 = 2: 1) was 4% of the mass of defatted soybean meal, the fermentation temp...
Embodiment 2
[0043]Primary fermentation: Take 1000g of soybean meal, add 100g of bran, adjust the pH to 6.0-6.5, and adjust the moisture content of the fermentation material to 65%. After sterilizing, cooling, and inoculating with strains, the amount of mixed strain fermented liquid added was 3% of the mass of defatted soybean meal, the fermentation temperature was 33°C, and the fermentation time was 92 hours. The acid-soluble protein content in fermented soybean meal was measured during and after the fermentation process.
[0044] Fermentation strains: strain 1 N1, strain 2 BS2, strain 3 Y3, strain mixing ratio N1 : BS2 : Y3 = 2:1:2.
[0045] Secondary fermentation: Take 1000g of the intermediate product after primary fermentation, adjust the pH to 6.0-6.5, and adjust the moisture content of the fermentation material to 65%. After sterilizing, cooling, and inoculating with strains, the amount of mixed strains fermented liquid (mixing ratio of strains N1:BS2:Y3 = 2:1:2) is 5% of the mass o...
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