Thelephoraganbajun Zang strain, Thelephoraganbajun Zang mycelium zinc polysaccharide fermented from Thelephoraganbajun Zang strain and application of Thelephoraganbajun Zang mycelium zinc polysaccharide

A technology of Zinc-enriched Dried Bacteria and Dried Bacteria, which is applied in the application field of Dried Bacteria Mycelia Zinc Polysaccharide and Dried Bacteria Mycelium Zinc Polysaccharide, which can solve the problems of difficult absorption, low bioavailability, gastrointestinal irritation, etc.

Active Publication Date: 2017-05-10
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems of poor effect, difficult absorption, gastrointestinal irritation, and low bioavailability of zinc supplement products in the above prior art, the present application provides a dry bark with high yield of mycelium zinc polysaccharide and high zinc content in zinc polysaccharide. bacteria( Thelephoraganbajun Zang) strain TG-1

Method used

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  • Thelephoraganbajun Zang strain, Thelephoraganbajun Zang mycelium zinc polysaccharide fermented from Thelephoraganbajun Zang strain and application of Thelephoraganbajun Zang mycelium zinc polysaccharide
  • Thelephoraganbajun Zang strain, Thelephoraganbajun Zang mycelium zinc polysaccharide fermented from Thelephoraganbajun Zang strain and application of Thelephoraganbajun Zang mycelium zinc polysaccharide
  • Thelephoraganbajun Zang strain, Thelephoraganbajun Zang mycelium zinc polysaccharide fermented from Thelephoraganbajun Zang strain and application of Thelephoraganbajun Zang mycelium zinc polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The acquisition of embodiment 1 stem bacteria strain

[0046] Collect 4 fresh fruiting bodies of D. spp. from different areas in Yunnan Province, disinfect the surface with 75% alcohol, rinse several times with sterile water, use sterilized tweezers to tear off small pieces of the internal tissue of D. spp. and inoculate them with chloramphenicol On the potato dextrose (PDA) medium plate, move it into a 24 ℃ incubator for cultivation. After the mycelium grows, it will be purified repeatedly until the pure culture of D. spp. is obtained.

[0047] Four strains of S. spp. were screened from 4 S. spp. fruiting bodies and named as TG-1, TG-2, TG-3 and TG-4 respectively.

Embodiment 2

[0048] Example 2 Analysis of Zinc-enriching Ability

[0049] Take 0.5 cm respectively 2 TG-1, TG-2, TG-3, and TG-4 were placed in PDA liquid medium and cultured on a shaking table at 24 °C for 7 days, and then transferred to PDA liquid containing different concentrations of zinc. In the medium (zinc concentration is 0, 50, 100, 150, 200, 300, 400, 500, 600, 700, 800 mg / L, ZnSO 4 ·7H 2 O), 5 repetitions for each gradient, 250 mL of liquid in a 500 mL Erlenmeyer flask, and shaking culture in a shaker at 24 °C for 10 days. After the fermentation, centrifuge (10000r / min, 10 min) to separate the mycelium of D. spp., and wash the mycelium with deionized water 3 times. The mycelium was dried in an oven at 55 ℃, and the biomass of the mycelium and the content of zinc in the mycelium were determined.

[0050] Figure 1~4 Respectively, TG-1, TG-2, TG-3, TG-4 fermentation medium zinc concentration and the relationship between mycelium biomass and zinc content. Appropriate concentra...

Embodiment 3

[0052] The zinc-rich liquid fermentation of embodiment 3 dried bacteria

[0053] Carry out fermenter culture experiments. ① Activation of bacteria. The bacteria TG-1, TG-2, TG-3, and TG-4 were respectively inoculated on the activated PDA medium plate, and cultured at 24 °C for 7 days. ② Seed cultivation. Take 0.5 cm respectively 2 The activated strain of D. spp. was inserted into zinc-rich PDA liquid medium (potato 200 g / L, glucose 20 g / L, magnesium sulfate 1 g / L, potassium dihydrogen phosphate 1.5 g / L, zinc concentration 150 mg / L , with ZnSO 4 ·7H 2 O), the liquid volume in a 500 mL Erlenmeyer flask was 300 mL, and then cultured in a shaker at 24 °C for 10 d. ③ Fermentation tank culture. Loading volume: 7.5 L; inoculum volume: 10 %; fermentation temperature: 24 ℃; stirring speed: 350 r / min; foam control: add two drops of defoamer after inoculation. ③ Determination of pH value and DO value during fermentation. The pH electrode and dissolved oxygen electrode are used t...

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Abstract

The invention relates to the technical field of mycelium zinc polysaccharides, in particular to a Thelephoraganbajun Zang strain TG-1. The collection number of the Thelephoraganbajun Zang strain TG-1 is CGMCC No.12977. A preparation method of a Thelephoraganbajun Zang mycelium zinc polysaccharide comprises the following steps: fermenting the Thelephoraganbajun Zang strain TG-1 in a liquid culture medium added with a zinc salt; separating mycelia; extracting the Thelephoraganbajun Zang mycelium zinc polysaccharide. The invention further discloses an application of the Thelephoraganbajun Zang mycelium zinc polysaccharide to preparation of a zinc supplement anti-aging health-care product. The Thelephoraganbajun Zang strain TG-1 has very high zinc element absorption and transformation capabilities, a high zinc element endurance capability and a strong mycelium growth capability, and can well enrich zinc elements during liquid fermentation. Thus, The Thelephoraganbajun Zang strain TG-1 has very important significance to the production of the Thelephoraganbajun Zang mycelium zinc polysaccharide and increase in the content of zinc in the zinc polysaccharide.

Description

technical field [0001] The invention relates to the technical field of mycelium polysaccharides, in particular to a strain of D. spp., and also relates to the zinc polysaccharide of D. spp. mycelia obtained by liquid fermentation of the strain, and the application of the zinc polysaccharide of D. spp. mycelium. Background technique [0002] Zinc is an essential trace element that performs the most physiological functions in the human body, and is widely involved in a series of biological processes such as enzyme activity regulation, substance metabolism, gene expression regulation, immune system regulation, and hormone regulation. However, zinc cannot be synthesized and stored in the body, and can only be obtained through daily supplementation, and the zinc obtained in the diet often cannot meet people's physiological needs. According to reports, 50% of the world's population is at risk of insufficient zinc intake. The nutritional imbalance caused by zinc deficiency is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/04A61K31/715A61P39/06A23L33/125A23L33/16C12R1/645
CPCA23V2002/00A61K31/715C12P19/04C12N1/145C12R2001/645A23V2200/30A23V2200/308
Inventor 郑岚杨俊慧杨艳蔡雷马润隆刘庆艾
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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