A dry bacterium strain and its fermented dry bacterium mycelium zinc polysaccharide and its application

A technology of Zinc-enriched Dried Bacteria and Dried Bacteria, which is applied in the application field of Dried Bacteria Mycelia Zinc Polysaccharide and Dried Bacteria Mycelium Zinc Polysaccharide, which can solve the problems of poor effect, gastrointestinal irritation, and difficulty in absorption, etc.

Active Publication Date: 2019-06-18
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems of poor effect, difficult absorption, gastrointestinal irritation, and low bioavailability of zinc supplement products in the above prior art, the present application provides a dry bark with high yield of mycelium zinc polysaccharide and high zinc content in zinc polysaccharide. bacteria( Thelephoraganbajun Zang) strain TG-1

Method used

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  • A dry bacterium strain and its fermented dry bacterium mycelium zinc polysaccharide and its application
  • A dry bacterium strain and its fermented dry bacterium mycelium zinc polysaccharide and its application
  • A dry bacterium strain and its fermented dry bacterium mycelium zinc polysaccharide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The acquisition of embodiment 1 stem bacteria strain

[0046] Collect 4 fresh fruiting bodies of D. spp. from different areas in Yunnan Province, disinfect the surface with 75% alcohol, rinse several times with sterile water, use sterilized tweezers to tear off small pieces of the internal tissue of D. spp. and inoculate them with chloramphenicol On the potato dextrose (PDA) medium plate, move it into a 24 ℃ incubator for cultivation. After the mycelium grows, it will be purified repeatedly until the pure culture of D. spp. is obtained.

[0047] Four strains of S. spp. were screened from 4 S. spp. fruiting bodies and named as TG-1, TG-2, TG-3 and TG-4 respectively.

Embodiment 2

[0048] Example 2 Analysis of Zinc-enriching Ability

[0049] Take 0.5 cm respectively 2 Dryingbacillus strains TG-1, TG-2, TG-3, and TG-4 were inserted into PDA liquid medium and cultured in a shaking table at 24°C for 7 days, and then transferred to PDA liquid containing different concentrations of zinc. In the medium (zinc concentration is 0, 50, 100, 150, 200, 300, 400, 500, 600, 700, 800 mg / L, ZnSO 4 ·7H 2 O), 5 repetitions for each gradient, 250 mL of liquid in a 500 mL Erlenmeyer flask, and shaking culture in a shaker at 24 °C for 10 days. After the fermentation, centrifuge (10000r / min, 10 min) to separate the mycelium of D. spp., and wash the mycelium with deionized water 3 times. The mycelium was dried in an oven at 55 ℃, and the biomass of the mycelium and the content of zinc in the mycelium were determined.

[0050] Figure 1~4 Respectively, TG-1, TG-2, TG-3, TG-4 fermentation medium zinc concentration and the relationship between mycelium biomass and zinc conte...

Embodiment 3

[0052] The zinc-rich liquid fermentation of embodiment 3 dried bacteria

[0053] Carry out fermenter culture experiments. ① Activation of bacteria. The bacteria TG-1, TG-2, TG-3, and TG-4 were respectively inoculated on the activated PDA medium plate, and cultured at 24 °C for 7 days. ② Seed cultivation. Take 0.5 cm respectively 2 The activated strain of D. spp. was inserted into zinc-rich PDA liquid medium (potato 200 g / L, glucose 20 g / L, magnesium sulfate 1 g / L, potassium dihydrogen phosphate 1.5 g / L, zinc concentration 150 mg / L , with ZnSO 4 ·7H 2 O), the liquid volume in a 500 mL Erlenmeyer flask was 300 mL, and then cultured in a shaker at 24 °C for 10 d. ③ Fermentation tank culture. Loading volume: 7.5 L; inoculum volume: 10 %; fermentation temperature: 24°C; stirring speed: 350 r / min; foam control: add two drops of defoamer after inoculation. ③ Determination of pH value and DO value during fermentation. The pH electrode and dissolved oxygen electrode are used t...

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Abstract

The invention relates to the technical field of mycelium zinc polysaccharides, in particular to a strain TG‑1 of D. spp. with a preservation number of CGMCC No. 12977. The preparation method of the zinc polysaccharide of the mycelia of D. shambrae: fermenting the strain TG‑1 of the shambarbacillus in a liquid medium added with zinc salts, separating the mycelia, and extracting the zinc polysaccharide of the mycelia of the shambarbacillus. The application of the zinc polysaccharide of the mycelia of D. dryum in the preparation of zinc-supplementing and anti-aging health care products. TG-1 strain TG-1 has good zinc absorption and transformation ability, zinc tolerance, and strong mycelium growth ability. During liquid fermentation, TG-1 can enrich zinc very well. Therefore, the TG-1 strain TG-1 of D. spp. is of great significance to the production of the zinc polysaccharide of D. spp. and the improvement of the zinc content in the zinc polysaccharide.

Description

technical field [0001] The invention relates to the technical field of mycelium polysaccharides, in particular to a strain of D. spp., and also relates to the zinc polysaccharide of D. spp. mycelia obtained by liquid fermentation of the strain, and the application of the zinc polysaccharide of D. spp. mycelium. Background technique [0002] Zinc is an essential trace element that performs the most physiological functions in the human body, and is widely involved in a series of biological processes such as enzyme activity regulation, substance metabolism, gene expression regulation, immune system regulation, and hormone regulation. However, zinc cannot be synthesized and stored in the body, and can only be obtained through daily supplementation, and the zinc obtained in the diet often cannot meet people's physiological needs. According to reports, 50% of the world's population is at risk of insufficient zinc intake. The nutritional imbalance caused by zinc deficiency is one ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04A61K31/715A61P39/06A23L33/125A23L33/16C12R1/645
CPCA23V2002/00A61K31/715C12P19/04C12N1/145C12R2001/645A23V2200/30A23V2200/308
Inventor 郑岚杨俊慧杨艳蔡雷马润隆刘庆艾
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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