Kit for detecting content of fibrinogen

A fibrinogen and detection kit technology, applied in the biological field, can solve problems such as unmentioned significance, and achieve the effects of high accuracy, good stability and wide detection range

Active Publication Date: 2017-05-10
BEIJING SICCEEDER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, few manufacturers in the market have mentioned "calcium-containing" reagent systems, and most p

Method used

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  • Kit for detecting content of fibrinogen
  • Kit for detecting content of fibrinogen
  • Kit for detecting content of fibrinogen

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0046] Example 1 Preparation and use method of the kit of the present invention

[0047] 1. Kit preparation

[0048] (1) Thrombin reagent (pH 7.4):

[0049]

[0050] (2) Imidazole buffer (pH 7.4):

[0051]

[0052] (3) Constant-value plasma: 3g / L.

[0053] 2. How to use the kit

[0054] The kit of the present invention is carried out on a coagulation automatic coagulation tester for routine inspection in hospital clinical laboratories, and its specific use method is as follows:

[0055] 1. Preparation of samples and reagents:

[0056] (1) Prepare the plasma sample to be tested: blood is collected from vein, 109mmol / L sodium citrate and whole blood are mixed uniformly in a ratio of 1:9. Centrifuge at 2500g for 15 minutes, and remove the plasma with a plastic pipette. The plasma sample must be tested within 4 hours.

[0057] (2) FIB thrombin reagent: accurately add distilled water to each bottle of thrombin according to the nominal amount of the bottle label, mix gently, and let it equilibra...

Example Embodiment

[0069] Example 2 Verification experiment of the stability of the optimal buffer system of the inventive kit:

[0070] In this experiment, in order to find the optimal ratio of the main components in the reagent system, the concentration of the thrombin reagent and imidazole buffer in the kit was compared and analyzed, and the three buffer systems of A, B, and C were tested. The FIB content of the reagents changed at 37°C, and the stability of the corresponding reagent system was evaluated.

[0071] Among them, the A, B, and C reagent formulations are shown in Table 2:

[0072] Table 2 Composition of reagent system with different concentrations:

[0073]

[0074] The specific experimental data are shown in Table 3 below:

[0075] Table 3 Stability of reagent system with different concentrations

[0076]

[0077]

[0078] The above experimental data show that the kit has better stability using the B reagent system, and the deviation of the FIB content detection value within 8 days does no...

Example Embodiment

[0079] Example 3 Ca in the kit of the invention 2+ Verification experiment for FIB quantitative accuracy:

[0080] This experiment is grouped as adding Ca to thrombin reagent 2+ No Ca in group and thrombin reagent 2+ Group, add Ca 2+ The group uses the B reagent system of Example 2 for detection, without Ca 2+ The group used reagents lacking calcium chloride based on the reagent system B of Example 2.

[0081] Each group randomly selects 10 reagents for FIB quantitative detection. The reference value of FIB in the control plasma is 2.8g / L. The average value of the detection value of each group and the deviation of the detection value from the reference value are calculated respectively to evaluate the Ca in the thrombin reagent. 2+ For the influence on FIB quantification, the relevant experimental data are shown in Table 4 below:

[0082] Table 4 Ca in thrombin reagent 2+ Impact on FIB quantitative accuracy

[0083]

[0084]

[0085] The above data shows that Ca is added to the thrombi...

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Abstract

The invention relates to the technical field of biology and particularly relates to a kit for detecting the content of fibrinogen. The kit comprises a thrombin reagent, an imidazole buffer solution and constant-value plasma, wherein the thrombin reagent comprises thrombin, imidazole, calcium chloride, mannitol, peptone, bovine serum albumin and sodium azide. A proper preparation scheme of the thrombin reagent and the imidazole buffer solution is determined and an in vitro diagnosis kit for clinical detecting the content of the FIB of human plasma in a hospital is researched. The thrombin reagent particularly contains Ca<2+> to reduce or supplement inhibited calcium ions in a clinical blood sample, an in vivo normal coagulation process is more ideally simulated and a more accurate data reference is provided for clinical detection. The basic performance of the kit reaches the industry standard requirements; the kit is high in accuracy, wide in detection range and good in stability and can be further popularized and applied to the market.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for measuring fibrinogen content by a Clauss method. Background technique [0002] Fibrinogen is a glycoprotein synthesized by the liver with blood coagulation function and is the precursor of fibrin. Increased fibrinogen levels are more common in diabetes, inflammatory stress response, and obesity; lower fibrinogen levels are more common in DIC and fibrinolysis. Severe impairment of liver function or congenital deficiency can reduce the concentration of plasma fibrinogen, and in severe cases, there may be a bleeding tendency. In addition, fibrinogen has also been used in studies of the pathogenicity of cardiovascular accidents. [0003] Fibrinogen is synthesized in the liver (1.7-5g / day) and megakaryocytes, and the reference value in plasma is 2-4g / l. Fibrinogen is composed of six chains, 2Aα, 2Bβ, and 2γ. Under the action of thrombin (factor IIa), the fibrinogen ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/75G01N2800/085G01N2800/32
Inventor 丁重辉闫君
Owner BEIJING SICCEEDER TECH CO LTD
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