Application of fingolimod hydrochloride in preparation of medicine for treating drug-induced liver injury
A fingolimod hydrochloride injury technology, applied in the application field of fingolimod hydrochloride, preparation of drugs for the treatment of drug-induced liver injury, can solve problems such as large side effects
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Embodiment 1
[0016] FTY720 attenuates APAP-induced liver injury
[0017] 1. Method: Source of kits required for the experiment: AST and ALT kits were purchased from Nanjing Jiancheng Bioengineering Institute, and FTY720 was purchased from Cayman Chemical Company. The experiment was divided into 4 groups: C57BL / 6 normal control group, FTY720 single treatment group, APAP single treatment group, FTY720 and APAP co-treatment group. The mice were starved overnight, and after 0.5 hour intraperitoneal injection of phosphate buffer solution or APAP (250 mg / kg ), FTY720 (5 mg / kg ) was injected intraperitoneally, the mice were anesthetized and sacrificed 6 hours and 24 hours later, and blood was collected.
[0018] 2. Results: see figure 1 , give mice intraperitoneal injection of APAP (250 mg / kg), at 6 hours and 24 hours can be observed in serum AST and ALT expression levels significantly increased; while FTY720 (5 mg / kg) and APAP co-treatment group, serum AST and ALT levels were significantly red...
Embodiment 2
[0020] FTY720 can reduce liver tissue necrosis induced by APAP
[0021] 1. Method: The mice were grouped as described in Example 1. The mice were starved overnight, and after 0.5 hours of intraperitoneal injection of phosphate buffered saline or APAP (250 mg / kg), FTY720 (5 mg / kg ) was injected intraperitoneally, respectively. After 6 hours and 24 hours, the mice were sacrificed under anesthesia, and the liver tissues were taken for H&E staining and TUNEL staining.
[0022] 2. Results: see figure 2 , After APAP treatment, the area of diffuse apoptotic necrosis in the center of the hepatic lobule of the mouse was significantly increased, and after 6 hours and 24 hours of treatment with FTY720, the area of necrosis in the mouse liver tissue was significantly reduced.
Embodiment 3
[0024] FTY720 can reduce the infiltration of inflammatory cells in liver tissue induced by APAP
[0025] 1. Method: The mice were grouped as described in Example 1, starved overnight, intraperitoneally injected with phosphate buffer saline or APAP (250 mg / kg) for 0.5 hours, and intraperitoneally injected with FTY720 (5 mg / kg), respectively, at 6 hours And 24 hours later, the mice were anesthetized, and the liver was removed. The liver tissue was fixed with 4% formaldehyde solution at 4 degrees for 72 hours and then sectioned. Histochemistry was stained with MPO and CD68.
[0026] 2. Results: see image 3 , After APAP treatment, the number of neutrophils and macrophages in the diffuse necrosis area of the mouse hepatic lobule increased significantly. Cell and macrophage numbers were significantly reduced.
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