Flushing fluid for collecting leukocyte or monocyte and collecting device

A monocyte and collecting device technology, applied in the field of medical devices, can solve the problems such as the inability of blood to be preserved and utilized, the amount of basal cells is small, and the self-transfusion, etc., to achieve the effects of simple structure, high survival rate, and avoidance of waste.

Inactive Publication Date: 2017-05-17
ZHANGJIAGANG GAOPINCHENG MEDICAL EQUIP TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in order to ensure the safety of clinical blood use and avoid blood transfusion reactions, people usually filter the leukocytes in the blood before transfusion, and most of the filtered leukocytes are discarded by people, and they are extracted clinically to treat malignant tumors and blood diseases. When the basal cells of immune cells such as diseases, the amount of basal cells obtained by traditional methods is small and the activity is low, and the blood after extraction of basal cells cannot be preserved and used or reinfused into the body, resulting in a great waste

Method used

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  • Flushing fluid for collecting leukocyte or monocyte and collecting device
  • Flushing fluid for collecting leukocyte or monocyte and collecting device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A rinsing solution for collecting white blood cells or monocytes, the rinsing solution includes the following components, sodium chloride, potassium chloride, sodium citrate, sodium dihydrogen phosphate, glucose, albumin and mannitol, wherein, Each 1000ml protection solution contains sodium chloride 7.02~8.95g; potassium chloride 0.13~0.21g; sodium citrate 2.52~3.57g; sodium dihydrogen phosphate 3.80~5.25g; glucose 3.05~4.25g; albumin 20~ 32g; Mannitol 3.22~4.02g; After mixing the above ingredients, fill the 1000ml volumetric flask with distilled water to dilute to 1000ml.

[0024] In this example, the 1000ml protection solution contains 7.83g of sodium chloride; 0.16g of potassium chloride; 2.92g of sodium citrate; 4.75g of sodium dihydrogen phosphate; 4.03g of glucose; 27.5g of albumin; In this example, the pH value of the flushing solution is between 7.3 and the osmotic pressure of the flushing solution is 318 mOsm / L.

[0025] The rinsing solution has an ion environ...

Embodiment 2

[0038] The difference between this example and Example 1 is that the blood collected in this example is from autologous, therefore, it is not necessary to centrifuge the leukocyte or mononuclear blood collection bag 5, see figure 2 As shown, there is no need for two parts, the empty plasma bag 52 and the blood cell culture solution bag 51, the blood transfusion tube can be directly connected to the leukocyte or mononuclear blood collection bag 5, and the leukocyte or monocyte blood can be removed by the blood transfusion needle 9. The blood is injected back into the body, and the obtained white blood cells or monocytes are purified, and then the clinically required immune cells are cultivated.

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Abstract

The invention discloses a flushing fluid for collecting leukocyte or monocyte and a collecting device; the flushing liquid is prepared from sodium chloride, potassium chloride, sodium citrate, monosodium phosphate, glucose, albumin, and mannitol, wherein every 1000 ml protection liquid contains 7.02-8.95 g of sodium chloride, 0.13-0.21 g of potassium chloride, 2.52-3.57 g of sodium citrate, 3.80-5.25 g of monosodium phosphate, 3.05-4.25 g of glucose, 20-32 g of albumin, and 3.22-4.02 g of mannitol; after mixing the components, distilled water is filled in 1000 ml volumetric flask and distilled to 1000 ml. the flushing fluid has similar plasma environment, pH environment and nutrient components as plasma, and is good for prolonging the survival time of the collected leukocyte or monocyte, and also can promote the collection amount of leukocyte or monocyte; the collecting device is simple in structure, and can collect leukocyte or monocyte on line; the collection efficiency is up to 90%; the survival rate is up to 80%; the blood after collecting can be stored or re-transformed to its main body; thereby effectively avoiding blood waste.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a washing liquid for collecting white blood cells or mononuclear cells and a collection device. Background technique [0002] As we all know, white blood cells, formerly known as white blood cells, are also called immune cells. They are a type of cells in the blood. They can help the body resist infectious diseases and foreign things as part of the immune system. They are an important blood cell in the blood. They are colorless under a microscope The nucleated cells are generally spherical in shape. According to the difference in shape, it can be divided into two categories: granular and non-granular. Granulocytes (granulocytes) that contain specially stained granules that can be distinguished by staining with Wright's stain are three types of granulocytes: neutrophils, eosinophils, and basophils; agranulocytes include monocytes cells and lymphocytes. The defense and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M1/36C12M1/00C12N5/078C12N5/0786
CPCA61M1/3692C12M47/04C12N5/0634C12N5/0645C12N2509/00
Inventor 杨海兵李剑平
Owner ZHANGJIAGANG GAOPINCHENG MEDICAL EQUIP TECH
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