Flushing fluid for collecting leukocyte or monocyte and collecting device
A monocyte and collecting device technology, applied in the field of medical devices, can solve the problems such as the inability of blood to be preserved and utilized, the amount of basal cells is small, and the self-transfusion, etc., to achieve the effects of simple structure, high survival rate, and avoidance of waste.
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Embodiment 1
[0023] A rinsing solution for collecting white blood cells or monocytes, the rinsing solution includes the following components, sodium chloride, potassium chloride, sodium citrate, sodium dihydrogen phosphate, glucose, albumin and mannitol, wherein, Each 1000ml protection solution contains sodium chloride 7.02~8.95g; potassium chloride 0.13~0.21g; sodium citrate 2.52~3.57g; sodium dihydrogen phosphate 3.80~5.25g; glucose 3.05~4.25g; albumin 20~ 32g; Mannitol 3.22~4.02g; After mixing the above ingredients, fill the 1000ml volumetric flask with distilled water to dilute to 1000ml.
[0024] In this example, the 1000ml protection solution contains 7.83g of sodium chloride; 0.16g of potassium chloride; 2.92g of sodium citrate; 4.75g of sodium dihydrogen phosphate; 4.03g of glucose; 27.5g of albumin; In this example, the pH value of the flushing solution is between 7.3 and the osmotic pressure of the flushing solution is 318 mOsm / L.
[0025] The rinsing solution has an ion environ...
Embodiment 2
[0038] The difference between this example and Example 1 is that the blood collected in this example is from autologous, therefore, it is not necessary to centrifuge the leukocyte or mononuclear blood collection bag 5, see figure 2 As shown, there is no need for two parts, the empty plasma bag 52 and the blood cell culture solution bag 51, the blood transfusion tube can be directly connected to the leukocyte or mononuclear blood collection bag 5, and the leukocyte or monocyte blood can be removed by the blood transfusion needle 9. The blood is injected back into the body, and the obtained white blood cells or monocytes are purified, and then the clinically required immune cells are cultivated.
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