Preparation method of low-dimensional carbon nanomaterial/quorum sensing quenching enzyme composite thin film material
A technology of carbon nanomaterials and quorum sensing, which is applied in biochemical equipment and methods, enzymes, lyases, etc., can solve the problems of not being able to solve the problem of rapid dissolution in aqueous solution, and achieves a simple and easy preparation method, easy preservation, and easy-to-prepare effects
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[0030] A method for preparing low-dimensional carbon nanomaterial / quorum sensing quenching enzyme composite film material, comprising the steps of:
[0031] 1) Preparation and selection of graphene oxide, graphene or carbon nanotubes:
[0032] Graphene oxide was prepared using Hummer's improved method. The natural flake graphite powder used is a 100 mesh powder with a purity of 99.999. The following materials are all calculated in parts by weight.
[0033] Mix 2 parts of natural flake graphite powder with 6 parts of sodium nitrate, slowly add 184 parts of concentrated sulfuric acid, fully stir at 4-6°C for 20-40 minutes; add 0.2 parts of potassium permanganate; add in batches within 1 hour at room temperature 8 parts potassium permanganate. Fully stir at 35±3°C for 1.5-2 hours to obtain a brown suspension;
[0034] Then slowly add 100 parts of water, and then add 10 parts of 30% H 2 o 2 and a mixed solution of 55mL ultrapure water, filtered to obtain a tan filter cake; ...
Embodiment 1
[0045] (1) Mix 2 parts of natural flake graphite powder with 6 parts of sodium nitrate according to the parts by weight, slowly add 184 parts of concentrated sulfuric acid, and fully stir for 30 minutes (5°C); add 0.2 parts of potassium permanganate. Within 1 h, 8 parts of potassium permanganate (20 °C) were added in portions. Stir well at 35±3°C for 2h to obtain a brown suspension. Slowly add 100mL100 parts of water, then add 10 parts of 30% H 2 o 2 and 55 mL of ultrapure water (45° C.), and filtered to obtain a tan filter cake. Wash the filter cake several times with 50 parts of dilute hydrochloric acid (3%, 45°C) and disperse it in 800 parts of water, then centrifuge, dialyze, ultrasonic (200W) for 10 hours, centrifuge the brown mixed solution for 30 minutes, freeze-dry in vacuum at -40°C for 24 hours, Store for later use.
[0046] (2) Preparation of graphene oxide solution: Disperse 1 mg of graphene oxide in 1 L of 0.2 M, pH-7.0 phosphate buffer solution, and ultrasoni...
Embodiment 2
[0058] (1) What the preparation of graphene oxide adopts is Hummer improved method, as described in embodiment 1.
[0059] (2) The preparation of graphene adopts graphene oxide reduction method (hydrazine hydrate reduction method): 0.1mg graphene oxide in the embodiment 1 is dispersed in the 1L aqueous solution, ultrasonic dispersion 40min, then add hydrazine hydrate (2ml, 50% w / v), mixed evenly and reacted under reflux conditions for 24h. After the reaction, the samples were filtered, separated, washed, and dried in a vacuum oven at 60°C.
[0060] (3) Preparation of graphene solution: Disperse 2 mg of graphene in 1 L of 0.2 M, pH-7.0 phosphate buffer solution, and ultrasonicate at room temperature for 30 min to obtain a 2.0 mg / L graphene solution.
[0061] (4) Preparation of quenching enzyme solution: select aminoacylase for quorum sensing quenching enzyme. Weigh 0.3mg of aminoacylase and add it into 1L of 0.2M, pH-7.0 phosphate buffer solution, stir at 0°C for 1.5h to obta...
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