Method for increasing content of cucurbitadienol in momordica grosvenori

A technology of cucurbitadienol and Luo Han Guo, applied in the field of plant biology, can solve the problems of low content and restrict the healthy development of Luo Han Guo industry, and achieve the effects of achieving accumulation, avoiding tea saponin precipitation, and improving adhesion

Inactive Publication Date: 2017-05-24
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that cucurbitadienol is an intermediate product of glucoside V biosynthesis. In the late development of Luo Han Guo fruit, cucurbitadienol is completely consumed and converted into glucoside V. However, cucurbitadienol only accounts for insufficient fruit weight. 15% in the pulp, and the content is extremely low, which seriously restricts the healthy development of the Luo Han Guo industry
There is no report in the prior art that methyl jasmonate increases the content of cucurbitadienol in Luo Han Guo

Method used

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  • Method for increasing content of cucurbitadienol in momordica grosvenori

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1, be that 2% ethanol aqueous solution is dissolved methyl jasmonate with volume fraction, be mixed with the ethanol mother liquor that concentration is 200mmol / L methyl jasmonate, and sterilize with the microporous membrane of 0.22 μm, the jasmine after sterilization Add the ethanol mother liquor of methyl jasmonate to MS solid medium, the amount of methyl jasmonate added is 200μmol / L, sterilize and cool to 24~26℃, wherein, MS solid medium includes: MS, 1.5mg / L L 6-benzylaminoadenine, 0.3mg / L indolebutyric acid, 3.5g / L agar, 30g / L sucrose, and 1.0g / L activated carbon;

[0028] Step 2. Inoculate the Luo Han Guo tissue cultured seedlings in the MS solid medium containing methyl jasmonate obtained in step 1, place them in a relative humidity of 60-66%, light intensity of 1300-1500Lux, light time of 8h / d, temperature of 21~ After culturing at 25°C for 30 days, the tissue-cultured seedlings were transplanted to the field for conventional field cultivation.

Embodiment 2

[0030] Dissolve methyl jasmonate in an aqueous ethanol solution with a volume fraction of 2%, and prepare a methyl jasmonate solution with a concentration of 200 μmol / L. After 25 days of pollination of Luo Han Guo, spray the methyl jasmonate solution on the surface of Luo Han Guo every day until the surface drips. Spray once in the morning, in the middle and in the evening, and spray continuously for 10 days.

Embodiment 3

[0032] Step 1, dissolving methyl jasmonate with a volume fraction of 2% ethanol aqueous solution is prepared with a concentration of ethanol mother liquor of 50mmol / L methyl jasmonate, and sterilized with a microporous membrane of 0.22 μm, the jasmine after sterilization Add the ethanol mother liquor of methyl jasmonate to MS solid medium, the amount of methyl jasmonate added is 50μmol / L, sterilize and cool to 24~26℃, wherein, MS solid medium includes: MS, 1.5mg / L L 6-benzylaminoadenine, 0.3mg / L indolebutyric acid, 3.5g / L agar, 30g / L sucrose, and 1.0g / L activated carbon;

[0033] Step 2. Inoculate the Luo Han Guo tissue cultured seedlings in the MS solid medium containing methyl jasmonate obtained in step 1, place them in a relative humidity of 60-66%, light intensity of 1300-1500Lux, light time of 8h / d, temperature of 21~ Culture at 25°C for 28 days;

[0034] Step 3, dissolve methyl jasmonate with a volume fraction of 2% ethanol aqueous solution, prepare a methyl jasmonate s...

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Abstract

The invention discloses a method for increasing the content of cucurbitadienol in momordica grosvenori. Jasmonic acid methyl ester is added in the tissue culture process and/or planting process of the momordica grosvenori. The method is capable of effectively increasing the yield of the cucurbitadienol in the momordica grosvenori, has the advantages of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or residue and the like, is capable of meeting large-scale production of the cucurbitadienol in the momordica grosvenori, and has relatively good practicability and high popularization values.

Description

technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for increasing the content of cucurbitadienol in Luo Han Guo. Background technique [0002] Luo Han Guo (Siraitia grosvenorii) is a precious medicinal and sweet plant unique to China. Its fruit is cool in nature and sweet in taste, and has the functions of clearing away heat and moistening lungs, relieving throat and opening sound, laxative and anticancer. The active ingredient glucoside V is one of the strongest non-sugar sweeteners in the world. It is 300-400 times higher than sugar and is widely used in food, health products and medicines. It is an ideal sugar substitute for diabetics, obese people and hypertensive patients. Studies have found that cucurbitadienol is an intermediate product of glucoside V biosynthesis. In the late development of Luo Han Guo fruit, cucurbitadienol is completely consumed and converted into glucosid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00A01G7/06
CPCA01G7/06A01G22/00A01H4/001
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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