Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of chicken adenovirus 4 type highly immunized yolk antibody

A technology of egg yolk antibody and chicken gland, which is applied in the field of preparation of chicken adenovirus type 4 hyperimmune egg yolk antibody, can solve problems such as ineffective treatment, achieve high antibody titer, long storage time, and reduce operating intensity Effect

Inactive Publication Date: 2017-05-24
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Pericardial effusion syndrome was initially thought to be caused by poisoning or malnutrition caused by mycotoxins, lipotoxicity, polychlorinated biphenyls, sodium chloride, algal toxins, etc. However, treatment based on these causes cannot be effectively cured

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of chicken adenovirus 4 type highly immunized yolk antibody
  • Preparation method of chicken adenovirus 4 type highly immunized yolk antibody
  • Preparation method of chicken adenovirus 4 type highly immunized yolk antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0020] Such as figure 1 As shown, a method for preparing chicken adenovirus type 4 hyperimmune egg yolk antibody, comprising: preparing a vaccine from an isolated strain, obtaining eggs from immune healthy flocks, sterilizing the eggs, extracting egg yolk, preparing, and subpackaging to obtain adenovirus 4 Type high immune egg yolk injection. Specific steps are as follows:

[0021] 1) Isolate virus strains to prepare vaccines: The virus samples this time were mainly liver, heart and other tissue samples collected from infected chickens in Xinzhou, Hubei. The samples were ground, frozen and thawed 3 times, and centrifuged at 12000g / min for 8 minutes. The supernatant was inoculated into the allantoic cavity of chicken embryos, and after three generations of transmission, the attenuated strain XH03 was verified according to the lesions...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a chicken adenovirus 4 type highly immunized yolk antibody. The preparation method comprises the steps: preparing vaccine by preparing a virulent strain with high immunity, preparing highly immunized eggs, sterilizing, collecting yolk and treating to obtain the adenovirus 4 type highly immunized yolk antibody. The chicken adenovirus 4 type highly immunized yolk antibody prepared by the method provided by the invention can effectively treat adenovirus, and can reduce operation strength and feeding cost in the clinical aspect; the chicken adenovirus 4 type highly immunized yolk antibody is convenient to transport and store and long in preservation time, and can maintain high antibody valence.

Description

technical field [0001] The invention belongs to the technical field of biological products for poultry, and in particular relates to a preparation method of chicken adenovirus type 4 hyperimmune yolk antibody. Background technique [0002] Avian adenoviruses belong to the genus Avian Adenoviridae, and can be divided into three groups according to their antigenicity. According to the serum neutralization reaction, group I avian adenoviruses contain 12 serotypes, and type 4 avian adenoviruses mainly infect The clinical and pathological symptoms of 3-5 week-old chickens are pericardial effusion syndrome, inclusion body hepatitis, nephritis, etc., and the fatality rate is 30-70%. Since adenovirus type 4 first broke out in Pakistan in 1987, it spread to some countries such as India, Japan, Mexico, Chile, and China, causing a lot of losses to the aquaculture industry. A large number of researchers have found that different strains are equally infected under rearing conditions, an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K16/08C07K16/02A61K39/42A61P31/20
CPCC07K16/081A61K2039/505C07K16/02
Inventor 罗青平郑培培邵华斌王红琳温国元张蓉蓉张腾飞汪宏才卢琴罗玲
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI