Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Paraffin sectioning method of roots of corydalis saxicola bunting

A technique of paraffin section and Coptis chinensis, which is applied in the field of histology, can solve the problems that the authenticity of medicinal materials cannot be identified, and the root section of Coptis chinensis is not clear enough, and achieves the effects of easy identification, clear structure and good effect.

Active Publication Date: 2017-05-24
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] An object of the present invention is to solve the problem that the root slices of Litectinus rhizome prepared by the prior art are not clear enough to be used for authenticity identification of medicinal materials, and to provide at least the advantages that will be described later

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paraffin sectioning method of roots of corydalis saxicola bunting
  • Paraffin sectioning method of roots of corydalis saxicola bunting
  • Paraffin sectioning method of roots of corydalis saxicola bunting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Fixing: After harvesting the roots of fresh Litectinus chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the vertical root midrib is cut across, and each piece is about 8mm long, wide 4mm, puts into the FAA fixative that volume concentration is prepared by 70% alcohol and fixes for 24 hours, and FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde volume The ratio is 90:5:5.

[0031] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 15 minutes, and stayed for 45 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 8...

Embodiment 2

[0040] (1) Fixing: After harvesting the roots of fresh Litectinus chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the midrib of the vertical root is cut across, each piece is about 5mm long and wide 3mm, puts into the FAA fixative that volume concentration is prepared by 70% alcohol and fixes for 24 hours, FAA fixative is 70% alcohol by volume concentration:glacial acetic acid:formaldehyde The ratio is 90:5:5.

[0041] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 15 minutes, and stayed for 55 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 85% alco...

Embodiment 3

[0050] (1) Fixing: After harvesting the fresh rock root, use tap water to wash off the dust on the surface, and absorb the water on the surface of the leaf. Then the midrib of the vertical root is cut across, each piece is about 10mm long and wide 5mm, puts into the FAA fixative that the volume concentration is prepared by 70% alcohol and fixes for 24 hours, and the FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde volume The ratio is 90:5:5.

[0051] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 18 minutes, and stayed for 60 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 85% alcohol,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a paraffin sectioning method of roots of corydalis saxicola bunting, which comprises sample fixing, dehydration, transparentizing, paraffin impregnation, embedding, sectioning and expansion, deparaffinage, dyeing and sealing. Before the transparentizing step, dehydrated roots of corydalis saxicola bunting are placed into sarranine solution with volume concentration of 0.8 to 1.2% to be dip-dyed for 8 to 12h; in the dyeing step, a slide with the roots of corydalis saxicola bunting is re-watered and then is placed into alcoholic solution to be subjected to color separation after re-watering, simultaneously, acetic acid with a volume ratio of 1: (800 to 1,000) is added into the alcoholic solution for color separation, and finally, the obtained product is placed into fast green solution with volume concentration of 0.8 to 1.2% to be dyed for 20 to 30 seconds. A root section prepared by the paraffin sectioning method disclosed by the invention is clearer in tissue, clear in profile root, and convenient in herbal medicine identification.

Description

technical field [0001] The present invention relates to the field of histology. More specifically, the present invention relates to a method for paraffin sectioning of the root of the root of the rhizome. Background technique [0002] Corydalis saxicola Bunting (Corydalis saxicola Bunting) is a plant of the Papaveraceae family, a perennial herb, distributed in Guangxi, Guangdong, Fujian, Hunan and other provinces of China. The whole herb is used as medicine, and the whole herb contains active ingredients such as dehydrocarvitin (petidine); it has significant antibacterial, anti-inflammatory, analgesic and strong stabilizing effects, and it has been observed to inhibit tumor cells; Hepatitis, liver cirrhosis, liver cancer and other diseases are well-known varieties of Zhuang Yao medicine. The plant distribution of Yanhuanglian is limited to limestone mountainous areas, and it is an endemic species of Shishan. Due to the poor habitat conditions, its natural reproduction rat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/286G01N1/30
Inventor 张占江缪剑华李翠郭晓云陈晓英胡营赵以民
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com