Composite collagen biological membrane and preparation method thereof

A collagen biology and collagen technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of incomplete closure of subdural space and dura mater defect, achieve controllable degradation, promote repair, and help effect on tissue growth

Active Publication Date: 2017-05-31
BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Factors such as trauma, tumor erosion, and dura mater shrinkage during surgery can cause dura mater defects, making it impossible to close the subdural space in situ. In addition, neurosurgery decompression usually requires expansion of the subdural space

Method used

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  • Composite collagen biological membrane and preparation method thereof
  • Composite collagen biological membrane and preparation method thereof
  • Composite collagen biological membrane and preparation method thereof

Examples

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preparation example Construction

[0041] In a second aspect, the present invention provides a method for preparing a composite collagen biofilm, comprising the following steps,

[0042] mixing the collagen with water, adding a first lactic acid solution and / or a first hydrochloric acid solution to the collagen to uniformly disperse the collagen to prepare a first slurry;

[0043] mixing the collagen with water, adding a second lactic acid solution and / or a second hydrochloric acid solution therein to disperse the collagen evenly to prepare a second slurry;

[0044] The mass fraction of collagen in the first slurry is greater than the mass fraction of collagen in the second slurry;

[0045]adjusting the pH value of the first slurry to 4-6.5, then vacuum defoaming the first slurry, then dehydrating and compressing, and then pre-freezing;

[0046] adjusting the pH value of the second slurry to 5.5-6.5, and then vacuum defoaming the second slurry;

[0047] pouring the second slurry over the already frozen first ...

Embodiment approach

[0063] As another improved embodiment of the present invention, the collagen is prepared by the following steps:

[0064] Slice the beef Achilles tendon first;

[0065] Then soak the sliced ​​bovine Achilles tendon in a phosphate buffer solution with a mass fraction of 0.005%-0.2% of ficin, and keep it at 4-15°C for 12-24h;

[0066] Then add an oxidizing agent to the phosphate buffer, and rinse the sliced ​​bovine Achilles tendon with water; the oxidizing agent is selected from at least one of chlorite and hydrogen peroxide;

[0067] Finally, soak the sliced ​​bovine Achilles tendon in a saline solution with a molar concentration of sodium hydroxide of 1-2mol / L, keep it at 4-15°C for 1-4 days, and then adjust the pH value of the saline solution to 4 -7. Collect the product, wash and dehydrate the product repeatedly to obtain collagen.

[0068] In the preparation method of the composite collagen biofilm provided by the present invention, the bovine Achilles tendon is first cu...

Embodiment 1-4

[0085] Example 1-4 Collagen Extraction

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Abstract

The invention discloses a composite collagen biological membrane and a preparation method thereof. Collagen in the composite collagen biological membrane is of a triple-helical structure; the composite collagen biological membrane is 0.2 to 0.9 cm in thickness, and is of a three-dimensional pore structure; the aperture of a multihole layer is 60 to 400 microns, and the aperture of a compact layer is 100 to 900 microns. The composite collagen biological membrane provided by the invention is formed by combining the compact layer with a relatively high collagen density and the multihole layer with relatively high porosity, so that the composite collagen biological membrane is high in mechanical strength, has suturing property and the advantages of biodegradability and low immunogenicity, and can be used as a substitute for an endocranium, a spinal membrane and the like.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a composite collagen biofilm and a preparation method thereof. Background technique [0002] Collagen, as a biopolymer, is the main component of animal connective tissue, and is also the most abundant and widely distributed functional protein in mammals, accounting for 25% to 30% of the total protein. It is closely related to the formation, maturation, and transmission of information between cells, as well as joint lubrication, wound healing, calcification, blood coagulation, and aging. Collagen is also one of the most critical raw materials in the biotechnology industry, and is widely used in medical materials, cosmetics, and food industries. In terms of spatial structure, collagen shows a special triple-helix winding structure, and three mutually independent collagen peptide chains rely on the hydrogen bonds formed between glycines to maintain the triple-helix win...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/58A61L27/50A61L27/24
CPCA61L27/24A61L27/50A61L27/56A61L27/58A61L2430/32C08L89/00
Inventor 富勇王丽君邓贺颖周月芬
Owner BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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