Collagen biological membrane and preparation method of collagen biological membrane

A collagen biology and collagen technology, applied in medical science, prosthesis, etc., can solve the problems of dura mater defect, in situ closure of the subdural space cannot be completed, etc., to promote repair, controllable degradation, and help tissue growth. Effect

Inactive Publication Date: 2017-05-10
BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Factors such as trauma, tumor erosion, and dura mater shrinkage during surgery can cause dura mater defects, making it impossible to close the subdural space in situ. In addition, neurosurgery decompression usually requires expansion of the subdural space

Method used

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  • Collagen biological membrane and preparation method of collagen biological membrane
  • Collagen biological membrane and preparation method of collagen biological membrane
  • Collagen biological membrane and preparation method of collagen biological membrane

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preparation example Construction

[0035] In a second aspect, the present invention provides a method for preparing a collagen biofilm, comprising the following steps,

[0036] Mix collagen with water, add lactic acid solution and / or hydrochloric acid solution to it, disperse the collagen evenly, and make a slurry;

[0037] adjusting the pH value of the slurry to 5.5-6.5, then vacuum defoaming the slurry, and then freeze-drying;

[0038] The collagen biofilm is obtained by physical and / or chemical cross-linking.

[0039] The preparation method of the collagen biofilm of the present invention comprises: mixing collagen with water, adding lactic acid solution and / or hydrochloric acid solution thereinto, making the collagen appear in the shape of elongated fibers, and the slurry is in a transparent state; the pH of the slurry is adjusted to 5.5-6.5 It is for the pH value of the collagen sponge formed after freeze-drying to be close to neutral, which is close to the pH value of the human body; choose lactic acid a...

Embodiment approach

[0049] As another improved embodiment of the present invention, the collagen is prepared by the following steps:

[0050] Slice the beef Achilles tendon first;

[0051] Then soak the sliced ​​bovine Achilles tendon in a phosphate buffer solution with a mass fraction of 0.005%-0.2% of ficin, and keep it at 4-15°C for 12-24h;

[0052] Then add an oxidizing agent to the phosphate buffer, and rinse the sliced ​​bovine Achilles tendon with water; the oxidizing agent is selected from at least one of chlorite and hydrogen peroxide;

[0053] Finally, soak the sliced ​​bovine Achilles tendon in a saline solution with a molar concentration of sodium hydroxide of 1-2mol / L, keep it at 4-15°C for 1-4 days, and then adjust the pH value of the saline solution to 4 -7. Collect the product, wash and dehydrate the product repeatedly to obtain collagen.

[0054] The preparation method of a collagen biofilm provided by the present invention comprises the steps of firstly cutting the bovine Achi...

Embodiment 1-4

[0071] Example 1-4 extracts collagen

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Abstract

The invention discloses a collagen biological membrane and a preparation method of the collagen biological membrane. The collagen biological membrane is characterized in that collagen in the collagen biological membrane has a triple-helical structure; the thickness of the collagen biological membrane is 0.2cm to 0.9cm; the collagen biological membrane has a three-dimensional pore diameter structure and the size of a pore diameter is 60mm to 300mm. The collagen biological membrane provided by the invention not only has high mechanical strength, but also has the advantages of absorbability and low immunogenicity; the collagen biological membrane can be used as a substitute good of endocranium, spinal membranes and the like.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a collagen biofilm and a preparation method thereof. Background technique [0002] Collagen, as a biopolymer, is the main component of animal connective tissue, and is also the most abundant and widely distributed functional protein in mammals, accounting for 25% to 30% of the total protein. It is closely related to the formation, maturation, and transmission of information between cells, as well as joint lubrication, wound healing, calcification, blood coagulation, and aging. Collagen is also one of the most critical raw materials in the biotechnology industry, and is widely used in medical materials, cosmetics, and food industries. In terms of spatial structure, collagen shows a special triple-helix winding structure, and three mutually independent collagen peptide chains rely on the hydrogen bonds formed between glycines to maintain the triple-helix winding struc...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/50A61L27/58
CPCA61L27/24A61L27/50A61L27/58A61L2300/412
Inventor 富勇王丽君邓贺颖周月芬
Owner BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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