An isothermal nucleic acid amplification chip

Abstract

The invention discloses an isothermal nucleic acid amplification chip, comprising upper and lower glass substrates which are bonded to each other and are relatively slidable, wherein injection holes A and B are formed in both sides of the upper glass substrate; a bonding surface is concavely engraved with a plurality of repeating units including a plurality of dog iron type flow channels, a plurality of bow type units and a plurality of rectangular units which are linearly distributed at intervals; an injection hole C, an outlet through hole A, an outlet through hole B and an outlet through hole C are also distributed in both sides of the repeating structure; the bonding surface of the lower glass substrate is concavely engraved with two groups of main flow channels and a plurality of repeating units corresponding to the main flow channels, including a plurality of circular units, a plurality of flow channels A and a plurality of flow channels B which are linearly distributed at intervals; and when the upper and lower glass substrates are bonded, the bonding surface can produce injection and reaction states. The operation is simple and convenient, different samples can be simultaneously amplified, the test operating strength can be reduced, the consumption of samples and reagents can be reduced, and the experimental cost can be reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a real-time recombinase polymerase isothermal nucleic acid amplification chip that can be applied in the fields of forensic medicine detection, environmental detection, food detection and the like. Background technique [0002] Microfluidic technology is one of the frontier fields of international high-tech nowadays. Its application in nucleic acid extraction, amplification, and detection research has achieved rapid development, and it has gradually become one of the most potential development directions of nucleic acid research. It has a chip with a micron-scale channel network structure, and realizes the integrated analysis and detection functions of biomedical and chemical laboratories through the manipulation and control of picoliter to nanoliter microfluidics in the chip. Slip chip (SlipChip) technology is a kind of microfluidic technology. It is composed of upper an...

AI Technical Summary

Problems solved by technology

[0004] However, in the current SlipChip field, due to the limitation of the chip structure, there are generally problems such as cumbersome operation and repeated control group experiments, which not only wastes manpower, but also wastes pharmaceutical reagents and increases the cost.

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