High bacterial load lactic acid bacteria preparation as well as preparation method and application thereof

A technology of lactic acid bacteria and bacterial counts, applied in the directions of biochemical equipment and methods, applications, chemical instruments and methods, etc., can solve the problems of high equipment requirements, complex operation, high fermentation cost, and achieve low fermentation cost, stable quality and high effect. significant effect

Inactive Publication Date: 2017-05-31
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology optimizes the production of Lactic Acid Bacterium (LAB) by culturing it under specific conditions such as temperature or pH levels. It also includes adding different chemical substances called sodium carbonate beforehand when producing certain types of microorganisms that are resistant against these factors. By doing this we aimed towards improving their ability to produce labeled probiotic strains without affecting them's natural environment.

Problems solved by technology

This patented technical problem addressed in this patents relating to developing efficient ways to promote healthy fish populations while minimizing environmental impact associated with traditional chemical treatments like pesticides that harm wildlife.

Method used

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  • High bacterial load lactic acid bacteria preparation as well as preparation method and application thereof
  • High bacterial load lactic acid bacteria preparation as well as preparation method and application thereof
  • High bacterial load lactic acid bacteria preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Fermentation preparation of a high bacterial content of Lactobacillus plantarum

[0040] 1. Materials and methods

[0041] Cultivation of Lactobacillus plantarum (Lactobacillus plantarum) 1A07806 preservation number CCTCC NO: M2013293: the starting strain was cultured in the order of slant, liquid seed, and liquid fermentation, and the slant and seed medium were conventional MRS medium. Fermentation medium formula: molasses 6%, brown sugar 2%, yeast powder 0.02%, peptone 0.5%, KH 2 PO 4 0.2%, FeSO 4 0.01%~0.02%, MnSO 4 0.005%~0.01%, CaCO 3 0.5%, the fermentation condition is 28~37℃, static culture for 36~48h, the final number of viable bacteria ≥10 in this way 9 CFU / mL;

[0042] By adding NaCO in batches 3 and CaCO 3 way to increase the number of viable bacteria in the final fermentation, the method is to add NaCO 3 Add water to dilute to 30% solution, then add 10% CaCO 3 , sterilized at 121°C for 20min, after cooling, when the pH of the fermentatio...

Embodiment 2

[0050] Example 2 Fermentation preparation of a high bacterial content Lactobacillus acidophilus

[0051] 1. Materials and methods

[0052] Lactobacillus acidophilus (Lactobacillus acidophilus) PL7 preservation number CCTCC NO: M2013294 culture: the starting strain was cultured in the order of slant, liquid seed, and liquid fermentation, and the slant and seed medium were conventional MRS medium. Fermentation medium formula (W / V): molasses 5%, brown sugar 3%, yeast powder 0.04%, peptone 0.4%, KH 2 PO 4 0.2%, CaCO 3 0.5%, molasses 3%, brown sugar 1%, yeast powder 0.6%, peptone 0.5%, KH 2 PO 4 0.2%, FeSO 4 0.01%~0.02%, MnSO 4 0.005%~0.01%, CaCO 3 0.5%, the fermentation condition is 28~37℃, static culture for 36~48h, the final number of viable bacteria ≥10 in this way 9 CFU / mL;

[0053] By adding NaCO in batches 3 and CaCO 3 way to increase the number of viable bacteria in the final fermentation, the method is to add NaCO 3 Add water to dilute to 30% solution, then ...

Embodiment 3

[0061] Example 3 Small-scale application test of the high-bacteria lactic acid bacteria preparation of the present invention in shrimp disease resistance and growth promotion

[0062] 1. Materials and methods

[0063] 1. Test product: the Lactobacillus plantarum bacterium liquid of embodiment 1, the total number of live bacteria is more than 8 billion / mL.

[0064] 2. Test prawns: divide the prawns into 2 groups, 250 tails in each group, and set 3 repetitions in each group.

[0065] 3. Test water body: The test water is sand filtered sea water.

[0066] 4. Test site: The test is carried out in a 500L glass fiber tank. The test cylinders were placed in an open place outdoors and placed randomly under natural conditions.

[0067] 5. Test method:

[0068] The test period is 45 days. The test is fed with conventional prawn feed. The test group is added with 1 kg of test bacteria per ton of feed to ensure that the number of viable bacteria in each gram of feed is ≥10 7 CFU / mL, ...

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Abstract

The invention discloses a high bacterial load lactic acid bacteria preparation as well as a preparation method and application thereof and relates to aquaculture. The high bacterial load lactic acid bacteria preparation contains at least one of lactobacillus plantarum, lactobacillus acidophilus and the like, wherein the content of the lactobacillus plantarum accounts for 50-60% of the total bacteria count percentage; and the content of the lactobacillus acidophilus accounts for 40-50% of the total bacteria count percentage. The lactobacillus plantarum and lactobacillus acidophilus can be uniformly mixed according to a ratio; and at least one of vitamin C, fructo-oligose, mannitol and the like is added to serve as a protective agent after mixing. When the existence form of the high bacterial load lactic acid bacteria preparation is a mixture of microbial powder and solid auxiliary materials, the preparation method comprises the following steps: mixing the fermentation liquor of lactobacillus plantarum and lactobacillus acidophilus, adding an additive, concentrating, performing freeze-drying, adding the solid auxiliary materials, and preparing tablets, powder and the like. The high bacterial load lactic acid bacteria preparation can be applied to preparation of agents for preventing and treating shrimp diseases, shrimp growth promoters, feed additives, water purification agents and the like.

Description

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Claims

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Application Information

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Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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