Detection method of hydrogen peroxide concentration based on non-enzymatic hydrogen peroxide sensor

Abstract

The invention relates to the field of electrochemistry, and in particular to a hydrogen peroxide concentration detection method based on an enzyme-free hydrogen peroxide sensor, which includes the following steps: (1) a three-electrode system is used for electro-depositing samarium hexacyanoferrate on the surface of a working electrode by cyclic voltammetry so as to obtain a samarium hexacyanoferrate-modified electrode; (2) the samarium hexacyanoferrate-modified electrode is immersed in silver nitrate solution, a silver / samarium hexacyanoferrate composite nanomaterial-modified electrode, i.e. the enzyme-free hydrogen peroxide sensor, is obtained by replacement reaction; (3) with PBS as a base solution for detection and the enzyme-free hydrogen peroxide sensor obtained in step (2) as a working electrode, a time-current method is adopted to detect hydrogen peroxide concentration. Because the detection method disclosed by the invention does not need enzyme during detection, the enzyme-free hydrogen peroxide sensor has the advantages of high stability, high repeatability, high specificity and interference resistance, high detection precision and low detection limit.

Description

technical field [0001] The invention relates to the field of electrochemistry, in particular to a hydrogen peroxide concentration detection method based on an enzyme-free hydrogen peroxide sensor. Background technique [0002] Hydrogen peroxide has multiple functions such as oxidation, bleaching, disinfection and sterilization. It can be miscible with water in any proportion. It is a strong oxidant. The aqueous solution is commonly known as hydrogen peroxide and is a colorless and transparent liquid. Its aqueous solution is suitable for medical wound disinfection, environmental disinfection and food disinfection. [0003] Rapid and accurate detection of hydrogen peroxide content is of great significance in food, pharmaceutical, biological and environmental analysis. Currently, there are many methods for detecting hydrogen peroxide, such as titration, spectrophotometry, and electrochemical methods. Among them, electrochemical methods, especially enzyme electrochemical biose...

AI Technical Summary

Problems solved by technology

However, because the enzyme is easily inactivated by various external factors, the enzyme electrochemical biosensor has poor stability and reproducibility when detecting the concentration of hydrogen peroxide, and its application range is narrow.