Method for quantitatively detecting concentration of folic acid in rat plasma through HPLC-MS/MS (high-performance liquid chromatography-mass spectrometry/mass spectrometry)

A quantitative detection, liquid mass spectrometry technology, applied in the field of biological analysis, can solve the problems that cannot satisfy the detection of large batches of samples, and achieve the effect of satisfactory detection, good reproducibility and high sensitivity

Active Publication Date: 2017-05-31
SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the detection of folic acid in the body mainly includes microbial assay, radioimmunoassay, chromatographic analysis, etc. These methods have certain limitations, and they cannot meet the detection of a large number of samples.

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  • Method for quantitatively detecting concentration of folic acid in rat plasma through HPLC-MS/MS (high-performance liquid chromatography-mass spectrometry/mass spectrometry)
  • Method for quantitatively detecting concentration of folic acid in rat plasma through HPLC-MS/MS (high-performance liquid chromatography-mass spectrometry/mass spectrometry)
  • Method for quantitatively detecting concentration of folic acid in rat plasma through HPLC-MS/MS (high-performance liquid chromatography-mass spectrometry/mass spectrometry)

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Embodiment Construction

[0024] The technical solution of the present invention will be further described in detail below in conjunction with specific embodiments and accompanying drawings. The following content is only for illustrating the present invention, but the present invention is not limited thereto.

[0025] 1 Instruments and reagents

[0026] Agilent 1260-6430 liquid chromatography mass spectrometry system (LC-MS / MS, Agilent Technologies Co., Ltd.). Including G1379B degasser, G1312B binary infusion pump, G1367E autosampler, G1330B temperature control module, G1316A column thermostat, 6430MS / MS system and Mass hunter data acquisition software. XL16K-T desktop high-speed refrigerated centrifuge, Hunan Xiangli Scientific Instrument Co., Ltd. Advantage A10Milli-Q ultrapure water machine, Merck Chemical Technology (Shanghai) Co., Ltd. Vortex Genius 3 vortex instrument, Guangzhou Yike Experimental Technology Co., Ltd.

[0027] Folic acid (purity 89.7%) and glibenclamide (purity 99.5%) were pur...

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Abstract

The invention discloses a method for quantitatively detecting concentration of folic acid in rat plasma through HPLC-MS / MS (high-performance liquid chromatography-mass spectrometry / mass spectrometry). Firstly, a rat plasma sample is treated through protein precipitation, and the content of the folic acid in rat plasma is detected with an HPLC-MS / MS method with glibenclamide as an interior standard. ESI positive ions and MRM (multiple reaction monitoring) are adopted in an MS acquisition method. With the adoption of the method, the linear relation of the folic acid at 10-1,000 ng / ml is good, and the recovery rate is in a range of 80.0%-95.4%; in-batch and batch-to-batch accuracy is 92.7%-111.4%, and the in-batch and batch-to-batch precision is 2.6%-8.3%. The method has good reproducibility, takes short analysis time, can meet the detection requirement of concentration of the folic acid in the rat plasma and can be used for pharmacokinetic study of folic acid tablets.

Description

technical field [0001] The invention belongs to the field of biological analysis, and in particular relates to a method for quantitatively detecting folic acid concentration in blood plasma by liquid mass spectrometry. Background technique [0002] Folic acid is a water-soluble vitamin composed of pteridine, p-aminobenzoic acid and L-glutamic acid. Folic acid is mainly involved in the synthesis and transformation of purine and pyrimidine nucleotides in the human body, in the formation of iron-containing hemoglobin, in protein metabolism, etc., and is an essential substance for cell growth and reproduction. Clinically, it can be used to prevent neural tube defects, and can also be used to treat chronic atrophic gastritis, inhibit bronchial squamous transformation, and prevent and treat coronary atherosclerosis, myocardial injury and myocardial infarction caused by hyperhomocysteinemia. Studies have shown that folic acid can also inhibit the expression of oncogenes in tumor c...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 戚敏王本伟王功霞王芳徐玲玲贾庆文马玉奎刘爱明逯海燕刘琳
Owner SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES
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