Paraffin sectioning method for Corydalis saxicola Bunting stems

A technology of paraffin sectioning and rock berberine, which is applied in the field of histology, can solve the problems of not clear enough root slices of rock berth, and the inability to identify the authenticity of medicinal materials, etc., to achieve clear structure, accurate embedding position, and obvious effect

Active Publication Date: 2017-06-06
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] An object of the present invention is to solve the problem that the root slices of Litectinus rhizome prepared by the prior art are not cl

Method used

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  • Paraffin sectioning method for Corydalis saxicola Bunting stems
  • Paraffin sectioning method for Corydalis saxicola Bunting stems
  • Paraffin sectioning method for Corydalis saxicola Bunting stems

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Fixing: After harvesting the stems of fresh Litectinus chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the midrib of the vertical stem is cut across, each piece is about 8mm long and wide 4mm, puts into the FAA fixative that the volume concentration is prepared by 70% alcohol and fixes for 24 hours, and the FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde The volume ratio is 90:5:5.

[0031] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 10 minutes, and stayed for 45 minutes; poured out a volume concentration of 80% alcohol, added a volume conc...

Embodiment 2

[0040] (1) Fixing: After harvesting the stems of fresh Litectinus chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the midrib of the vertical root is cut across, each piece is about 5mm long and 3mm wide, and put into the FAA fixative prepared by 70% alcohol by volume concentration to fix for 24 hours. The FAA fixative heavy alcohol: glacial acetic acid: formaldehyde volume ratio is 90:5 :5.

[0041] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 15 minutes, and stayed for 55 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 85% alcohol, vacuumed for 10...

Embodiment 3

[0050] (1) Fixing: After harvesting the stems of fresh Litectin chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the midrib of the vertical root is cut across, each piece is about 10mm long and 5mm wide, and put into the FAA fixative prepared by 70% alcohol by volume concentration to fix for 24 hours. The FAA fixative heavy alcohol: glacial acetic acid: formaldehyde volume ratio is 90:5 :5.

[0051] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 10 minutes, and stayed for 60 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 85% alcohol, vacuumed for 10 ...

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Abstract

The invention discloses a paraffin sectioning method for Corydalis saxicola Bunting stems. The paraffin sectioning method comprises the following steps: sample fixation, dehydration, transparency treatment, wax dipping, embedding, sectioning and slice expansion, dewaxing, dyeing and slice sealing. The paraffin sectioning method specifically comprises the following steps: before the transparency treatment, putting dehydrated Corydalis saxicola Bunting stems into a 0.8%-1.2% sarranine solution, and carrying out dip dyeing for 8-12 hours; after the dewaxing, carrying out rehydration on a slide carrying the Corydalis saxicola Bunting stems, putting the Corydalis saxicola Bunting stems into an alcohol solution, carrying out color separation, simultaneously adding acetic acid into the alcohol solution for the color separation in a volume ratio of 1 to (800-1000), finally, putting the Corydalis saxicola Bunting stems into a 0.8%-1.2% fast green solution, and carrying out dyeing for 10-20 seconds. By virtue of the paraffin sectioning method disclosed by the invention, a clear Corydalis saxicola Bunting stem structure can be sectioned, a specimen can be stored for a long time for use, is a permanent microscopic slide specimen and can be applied to microscopic identification of Corydalis saxicola Bunting medicinal materials for market normalization.

Description

technical field [0001] The present invention relates to the field of histology. More specifically, the present invention relates to a method for paraffin sectioning of the stem of Litectinum rhizome. Background technique [0002] Corydalis saxicola Bunting (Corydalis saxicola Bunting) is a plant of the Papaveraceae family, a perennial herb, distributed in Guangxi, Guangdong, Fujian, Hunan and other provinces of China. The whole herb is used as medicine, and the whole herb contains active ingredients such as dehydrocarvitin (petidine); it has significant antibacterial, anti-inflammatory, analgesic and strong stabilizing effects, and it has been observed to inhibit tumor cells; Hepatitis, liver cirrhosis, liver cancer and other diseases. It is a famous Zhuang Yao medicine variety. The plant distribution of Yanhuanglian is limited to limestone mountainous areas, and it is a unique species of Shishan. Due to the poor habitat conditions, its natural reproduction rate is very l...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N1/28
CPCG01N1/286G01N1/30G01N2001/2873
Inventor 李翠张占江陈晓英郭晓云胡营赵以民
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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