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Marker composition and detection kit for liver cancer detection

A technology of markers and kits, applied in the field of marker combinations and detection kits for liver cancer detection, to achieve the effect of improving sensitivity and specificity, high sensitivity and specificity

Active Publication Date: 2017-06-09
合众赛博(北京)科技发展有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Serological examination mainly detects tumor markers in serum, and currently mainly relies on the detection of alpha-fetoprotein (AFP), but the sensitivity of AFP in small liver cancer is only about 40%

Method used

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  • Marker composition and detection kit for liver cancer detection
  • Marker composition and detection kit for liver cancer detection
  • Marker composition and detection kit for liver cancer detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1: Collection and processing of serum from different groups of people

[0080] In the Cancer Hospital of Tianjin Medical University, 60-300 cases of peripheral blood were collected from liver cancer patients, hepatitis B patients and normal subjects respectively. In the experiment, the serum of 288 patients with liver cancer, 87 patients with hepatitis B, 80 patients with liver cirrhosis and 241 normal patients were collected.

[0081] Within 1 hour of peripheral blood collection, centrifuge at 3000g for 5-10 minutes, transfer the serum into a new centrifuge tube, and store in a -80°C refrigerator.

Embodiment 2

[0082] Example 2: Diagnostic application of markers of the present invention or combinations thereof

[0083] The concentrations of AFP, AFU, GPC3, GGT2, and HGF in the serum to be tested were detected by ELISA using a commercially available kit (details are shown below) according to the manufacturer's instructions.

[0084]

[0085] 1) Evaluation indicators and selection of diagnostic tests

[0086] Sensitivity (true positive rate, Sensitivity), specificity (true negative rate, Specificity), positive predictive value (PPV), negative predictive value (NPV) were used to evaluate the diagnostic effect of the tested indicators:

[0087] Sensitivity=TP / (TP+FN)×100%

[0088] Specificity=TN / (TN+FP)×100%

[0089] Positive predictive value = TP / (TP+FP) × 100%

[0090] Negative predictive value = TN / (TN+FN) × 100%

[0091] Correct index (Youden index) = (sensitivity + specificity) - 100%

[0092] Among them, TN (true negative) = true negative; FP (false positive) = false positi...

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Abstract

The invention relates to a marker or a composite thereof for liver cancer detection, a reagent and a detection kit for detecting liver cancer, and application of the marker or the composite thereof, the reagent and the detection kit in detecting liver cancer and distinguishing patients with liver cancer, patients with liver cirrhosis and / or healthy people.

Description

technical field [0001] The present invention relates to markers or combinations thereof for liver cancer detection, reagents and kits for detecting them, and their use in liver cancer detection and in distinguishing liver cancer patients from hepatitis B patients, liver cirrhosis patients and / or healthy persons . Background technique [0002] Primary liver cancer (PLC, referred to as liver cancer) is one of the most common clinical malignant tumors. It has an insidious onset, rapid invasion and growth, easy recurrence after treatment, and extremely poor prognosis. It is called the "king of cancers". About half of the liver cancer patients in the world are concentrated in China. About 110,000 people die of liver cancer in China every year, ranking second in the death rate of malignant tumors. At present, surgical resection and liver transplantation are the most effective treatment methods for liver cancer, but the vast majority of liver cancer patients are in the middle and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574G01N33/573
CPCG01N33/573G01N33/57438G01N33/57484G01N33/68G01N2333/47G01N2333/4722G01N2333/4753G01N2333/9108G01N2333/924
Inventor 张宁郭华罗艺陈丽维
Owner 合众赛博(北京)科技发展有限责任公司
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