PCR primers, probes and method for detecting III type maloccluded CPDHB3 gene mutation

Abstract

The invention relates to the technical field of genomic DNA mutation detection, and in particular relates to PCR primers, probes and a method for detecting III type maloccluded CPDHB3 gene mutation. The PCR primers comprise two primers (one pair) for amplifying a polymorphic site of a PCDHB3 gene; the probes comprise two probes for detecting the polymorphic site of the PCDHB3 gene; and each of the probes comprises 13-25 sequences of basic groups, the 5' end has a fluorescent group label, and the 3' end has an MGB group label. The PCR primers are good in specificity; and the probes are good in specificity and sensitive and precise in detection. The fluorescent quantitative PCR detection method can detect the polymorphism of the III type maloccluded CPDHB3 gene, and is good in accuracy, high in sensitivity, simple in step, convenient in operation and control and high in detection efficiency.

Description

technical field [0001] The invention relates to the technical field of human genome DNA mutation detection, in particular to a PCR primer, a probe and a method for detecting type III malocclusion PCDHB3 gene mutation. Background technique [0002] Class III malocclusion refers to the mesial position of the mandible and the lower dental arch, and the mesial relationship between the molars. It can be divided into three types of skeletal and three types of dental malocclusions. It is a common developmental deformity in clinical practice. It is a variety of craniofacial developmental abnormalities and dentition abnormalities, including: anterior overbite, crossbite, open and close, maxillary retraction or too small, mandibular protrusion or hypertrophy. The prevalence of Class III malocclusion in the Chinese population is very high. According to the survey results conducted by Beijing Medical University in 2000, the prevalence rates of Class III malocclusion in deciduous dentiti...

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Problems solved by technology

Firstly, it affects the function of the oral cavity, causing mastication dysfunction, abnormal swallowing, and indigestion in severe cases; secondly, it affects the language function, the more obvious the anti-coverage, the smaller the repetition, the more obvious the abnormal speech function; thirdly, it affects the appearance, manifested as facial features In the middle 1 / 3 of the depression and mandibular protrusion deformity, with the severity of the malocclusion, the face presents a crescent shape; what is more serious, it also leads to the patient's mental inferiority complex and isolation, and even causes serious morbidity. mental disorder

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