Method for evaluating throat stimulation degree of traditional Chinese medicine
An evaluation method, a throat-stimulating technology, applied in the direction of testing pharmaceutical preparations, material inspection products, etc., can solve problems such as toxicity, and achieve the effect of improving accuracy and evaluating objectively
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[0031] 1 Experimental materials
[0032] 1.1 Drugs and reagents
[0033] Preparation of licorice juice: Take 15 g of clean licorice, add 150 mL of water to decoct for 30 min, filter, continue to add 120 mL of water to decoct for 30 min, filter, and combine the concentrate to 150 mL. That is, 1 g of licorice is equivalent to 10 mL of licorice juice concentrate.
[0034] Preparation of different elution parts of polygala macroporous resin: Weigh 500 g of polygala medicinal material, extract twice with 70% ethanol under reflux, each time the amount of 70% alcohol is 8 times the weight of medicinal material, the extraction time is 2 h for the first time, and 2 h for the second time. Time 1 h. The two extracts were combined, filtered, concentrated under reduced pressure at 60°C, and the solvent was recovered to obtain a dark brown extract. Take the extract, add appropriate amount of distilled water to suspend, extract with n-butanol (drug solution: n-butanol = 1:1), recover the ...
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