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A flunixin meglumine monoclonal antibody hybridoma cell line yy and its application

A flunixin meglumine, hybridoma cell line technology, applied in instruments, peptides, immunoglobulins, etc., can solve the problems of low sensitivity, difficult to obtain accurate results, and many interfering substances, and achieve good specificity, good The effect of detection sensitivity

Active Publication Date: 2020-05-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to effectively supervise and monitor the clinical use of flunixin meglumine in veterinary medicine, it is necessary to find a method with good specificity and high sensitivity. However, current detection methods such as thin-layer chromatography, gas chromatography, and liquid chromatography etc., the separation and purification process is lengthy, the sensitivity is low, and there are many interfering substances, it is difficult to obtain accurate results

Method used

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  • A flunixin meglumine monoclonal antibody hybridoma cell line yy and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Embodiment 1 Preparation of hybridoma cell line YY

[0019] (1) Preparation of complete antigen: Weigh 2.2 mg FM, 2.6 mg 1-ethylcarbodiimide hydrochloride, 1.6 mg N-hydroxysuccinimide, and dissolve with 400 μL anhydrous N,N-dimethylformaldehyde The amide was dissolved and stirred at room temperature for 6-8h (referred to as solution A). Take 10 mg of bovine serum albumin BSA (the molar ratio of FM to BSA is 30:1), and dissolve it with 4 mL of boric acid buffer solution (called solution B). At room temperature, add solution A to solution B dropwise, and react overnight at room temperature to obtain the conjugate FM-BSA mixture, and separate the complete antigen and unconjugated small molecule hapten by dialysis. Preparation of coated original FM-OVA: Weigh 1.6mg FM, 1.9mg 1-ethylcarbodiimide hydrochloride, 1.2mg N-hydroxysuccinimide, add 300μL anhydrous N,N-dimethyl Dissolve the formamide and stir at room temperature for 6-8 hours (referred to as liquid A). Weigh 5 mg...

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Abstract

Provided is a flunixin meglumine monoclonal antibody hybridoma cell strain YY, which has been collected in the China General Microbiological Culture Collection Center (referred to as CGMCC), with the accession number being CGMCC No.13096. Also provided are a monoclonal antibody secreted by the hybridoma cell strain YY, and an application of the monoclonal antibody. Also provided are a method for preparing a flunixin meglumine antigen, the antigen prepared by the method, and an application of the antigen.

Description

technical field [0001] The invention relates to a flunixin meglumine monoclonal antibody hybridoma cell line YY and an application thereof, belonging to the field of food safety immunoassay. Background technique [0002] Flunixin meglumine (flunixin meglumine, FM) is a new type of non-steroidal animal-specific antipyretic and analgesic drugs, of which, flunixin (flunixin, molecular formula C 14 h 11 f 3 N 2 o 2 ) is a special structure of a class of non-steroidal anti-inflammatory analgesic antipyretic drugs, meglumine (meglumine, molecular formula C 7 h 17 NO 5 ) mainly acts as a stabilizer, and the two appear in a fixed ratio (1:1, that is, one molecule of flunicin combined with one molecule of meglumine), which is FM. In veterinary medicine, it is often used to relieve horses' visceral colic, pain caused by muscle and bone disorders, and anti-inflammation; to control acute inflammation caused by various diseases and infections in cattle, such as laminitis, arthriti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/44G01N33/577G01N33/94C12R1/91
CPCC07K16/44G01N33/577G01N33/9486
Inventor 胥传来林璐匡华徐丽广马伟刘丽强吴晓玲宋珊珊胡拥明
Owner JIANGNAN UNIV