Composition for detecting colla corii asini content in compound colla corii asini preparation, kit and detection method thereof

A compound donkey-hide gelatin and composition technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of quantitative detection of donkey skin source components, discounts on accuracy and reliability, and supervision of unfavorable market donkey-hide gelatin products.

Active Publication Date: 2017-06-20
SHAN DONG DONG E E JIAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The two reported methods are unable to carry out more accurate quantitative detection of donkey-hide gelatin-derived components, but to determine the authenticity of donkey-hide gelatin by detecting whether donkey-hide gelatin contains donkey components and excluding other animal-derived components; but the donkey-hide gelatin Adulteration may be varied, so there will be a variety of animal-derived ingredients that need to be excluded. There are important flaws in using the exclusion method to identify the authenticity of donkey-hide gelatin, and its accuracy and reliability will be greatly reduced. This situation is very unfavorable to the market for donkey-hide gelatin. product regulation

Method used

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  • Composition for detecting colla corii asini content in compound colla corii asini preparation, kit and detection method thereof
  • Composition for detecting colla corii asini content in compound colla corii asini preparation, kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1Materials and reagents

[0034] 1) Material

[0035] A variety of pure glues, including donkey skin, horse skin glue, yellow gelatin, pig skin glue, sheep skin glue, fish skin glue (reference test substrate), respectively, donkey skin, horse skin, cowhide, pig skin, sheep skin and fish skin It is obtained after decoction, concentration and drying.

[0036] Polypeptide I (shown in SEQ ID NO. 1), polypeptide II (shown in SEQ ID NO. 2), and polypeptide III (shown in SEQ ID NO. 3) were synthesized by a biological company.

[0037] Compound donkey-hide gelatin preparation sample: extract the concentrated solution of plant medicinal materials in the production process of compound donkey-hide gelatin, respectively add the above pure product gum, and add 1.0g pure product gum sample for every 9g of plant medicinal material extraction concentrated solution to make compound donkey-hide gelatin paste containing donkey-hide gelatin ( Preparation 1), fake donkey-hide gelatin containing ho...

Embodiment 2

[0057] 1Materials and reagents

[0058] 1) Material

[0059] Polypeptide I (shown in SEQ ID NO. 1), polypeptide II (shown in SEQ ID NO. 2), and polypeptide III (shown in SEQ ID NO. 3) were synthesized by a biological company.

[0060] The mixed gum samples were prepared by adding different quality donkey-hide gelatin samples to the pure product gum samples in Example 1, including yellow gelatin containing 15% donkey-hide gelatin, yellow gelatin containing 30% donkey-hide gelatin, and yellow gelatin containing 60% donkey-hide gelatin.

[0061] Compound donkey-hide gelatin preparation samples: extract the concentrated solution of plant medicinal materials in the production process of compound donkey-hide gelatin slurry, add the above mixed gum samples respectively, and add 1.0g mixed gelatin sample to each 9g plant medicinal extract concentrated solution to make 3 fake compound donkey-hide gelatin slurry. They were named Formulation 1, Formulation 2, and Formulation 3. The donkey-hide g...

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PUM

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Abstract

The invention discloses a composition for detecting colla corii asini content in a compound colla corii asini preparation, a kit and a detection method thereof. The composition is prepared from polypeptide I, polypeptide II, polypeptide III and a reference substance detection substrate, wherein an amino acid sequence of the polypeptide I is as shown in SEQ ID NO.1; an amino acid sequence of the polypeptide II is as shown in SEQ ID NO.2; amino acid sequence of the polypeptide III is as shown in SEQ ID NO.3; the reference substancedetection substrate is a fish skin glue. A detection value of the polypeptide I minus a detection value of the polypeptide II, and a detection value of the polypeptide III is utilized for correcting a detection error, so that the detection of the colla corii asini content in the compound colla corii asini preparation is realized. The method has the advantages of content detection accuracy and simplicity in operation, and has a wide application prospect in the quality monitoring field of the compound colla corii asini preparation.

Description

Technical field [0001] The invention relates to a composition and a kit for detecting the donkey-hide gelatin content in a compound donkey-hide gelatin preparation, and also includes a method for detecting the donkey-hide gelatin content in a compound donkey-hide gelatin preparation by using the composition or the kit. The invention belongs to the technical field of medicine and food detection. Background technique [0002] As a traditional precious Chinese medicine, donkey-hide gelatin has a history of consuming thousands of years. It has the effects of nourishing blood and yin, moisturizing dryness, and stopping bleeding. According to the Chinese Pharmacopoeia, donkey-hide gelatin is a solid glue made by decoction and concentration of the dried skin or fresh skin of the equine donkey Equus asinus L.. Pure donkey-hide gelatin must come from 100% donkey skin to ensure the effectiveness of the product. From the perspective of raw material taxonomy, donkeys belong to the genus Equ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/02G01N30/06
Inventor 郭尚伟郝向慧段小波徐云鹏王静王玉娇石永坚周祥山
Owner SHAN DONG DONG E E JIAO
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