A kind of micro-propagation method of long-beaked Ranunculus Alisma

A technology of micropropagation and culture medium, applied in the field of horticulture and forestry

Active Publication Date: 2019-04-16
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few reports on the tissue engineering seedlings of Alisma long-beaked Ranunculus

Method used

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  • A kind of micro-propagation method of long-beaked Ranunculus Alisma
  • A kind of micro-propagation method of long-beaked Ranunculus Alisma
  • A kind of micro-propagation method of long-beaked Ranunculus Alisma

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1. Materials and methods

[0026] 1.1 Experimental materials

[0027] Alisma long-beaked buttercup seedlings were provided by Dr. Shi Congguang of Zhejiang Academy of Forestry Sciences, and were cultivated in an artificial climate chamber in our laboratory. The experimental materials used the leaves, petioles and stems with meristems of the plant.

[0028] 1.2 Medium and culture conditions

[0029] 1.2.1 MS medium formula

[0030] MS medium is used as the basic medium, and its chemical composition and dosage are as follows (concentration unit of working solution: mg / L): Macroelement: NH 4 NO 3 (1650), KNO 3 (1900), CaCl 2 2H 2 O(440), MgSO 4 7H2O (370), KH 2 PO 4 (170); trace elements: KI (0.83), H 3 BO 3 (6.2), MnSO 4 4H 2 O(22.3), ZnSO 4 ·7H 2 O(8.6), Na 2 MoO 4 2H 2 O (0.25), CuSO 4 ·5H 2 O (0.025), CoCl 2 ·6H 2 O (0.025); iron salt: FeSO 4 ·7H 2 O (27.8), Na 2 -EDTA·2H 2 O (37.3); organic substances: inositol (100), niacin (0.5), pyridoxine...

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Abstract

The invention relates to a micro-propagation method of ranalisma rostratum, and belongs to the technical field of gardening forestry. The micro-propagation method comprises the following steps of using cleaned ranalisma rostratum as an explant to sterilize, so as to obtain a sterile seedling; selecting a micro-propagation organ on the sterile seedling to perform rooting culture, so as to obtain a tissue culture seedling; transplanting. The micro-propagation method has the advantages that after the micro-propagation method is used for culturing the ranalisma rostratum, the culture speed is high, the survival rate is high, and the like.

Description

technical field [0001] The invention relates to a micropropagation method for long-beaked buttercup Alisma alisma, belonging to the technical field of horticulture and forestry. Background technique [0002] Long-billed Buttercup Alisma ( Ranalisma rostratum ) is an endangered aquatic herbaceous plant belonging to the genus Alisma genus of the family Alismaceae. It is produced in Zhejiang, Jiangxi, Hunan, Vietnam and Malaysia in China. At present, only scattered small groups have been found in Jiangxi and Hunan in my country, and the number of groups and Individuals are still shrinking, indicating that this plant is in a critically endangered state. At present, there are few reports on the tissue engineering seedlings of Alisma long-beaked Ranunculus. How to expand its population and protect the plant is an urgent problem to be solved. [0003] Based on this, this application is made. Contents of the invention [0004] Aiming at the present situation of Alisma long-beak...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 郭万里杨佳瑶梁宗锁石从广向倩倩杨宗岐祁哲晨丁先锋
Owner ZHEJIANG SCI-TECH UNIV
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