A kind of micro-propagation method of long-beaked Ranunculus Alisma
A technology of micropropagation and culture medium, applied in the field of horticulture and forestry
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[0025] 1. Materials and methods
[0026] 1.1 Experimental materials
[0027] Alisma long-beaked buttercup seedlings were provided by Dr. Shi Congguang of Zhejiang Academy of Forestry Sciences, and were cultivated in an artificial climate chamber in our laboratory. The experimental materials used the leaves, petioles and stems with meristems of the plant.
[0028] 1.2 Medium and culture conditions
[0029] 1.2.1 MS medium formula
[0030] MS medium is used as the basic medium, and its chemical composition and dosage are as follows (concentration unit of working solution: mg / L): Macroelement: NH 4 NO 3 (1650), KNO 3 (1900), CaCl 2 2H 2 O(440), MgSO 4 7H2O (370), KH 2 PO 4 (170); trace elements: KI (0.83), H 3 BO 3 (6.2), MnSO 4 4H 2 O(22.3), ZnSO 4 ·7H 2 O(8.6), Na 2 MoO 4 2H 2 O (0.25), CuSO 4 ·5H 2 O (0.025), CoCl 2 ·6H 2 O (0.025); iron salt: FeSO 4 ·7H 2 O (27.8), Na 2 -EDTA·2H 2 O (37.3); organic substances: inositol (100), niacin (0.5), pyridoxine...
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