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Preparation method and application of wolfberry leaf oligosaccharide and acidic polysaccharide

A technology of acidic polysaccharides and wolfberry leaves, applied in the field of medicine, to achieve good application effect, good economic and social benefits, and simple preparation method

Inactive Publication Date: 2019-08-13
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Ningxia Lycium barbarum Lycium barbarum The dry leaves of L., commonly known as Tianjingcao, "Compendium of Materia Medica" claim that it has the effects of "removing annoyance, benefiting will, nourishing five labors and seven injuries, strengthening the heart, removing heat and poison, dispelling sores and swelling, removing wind and improving eyesight". It shows that it mainly contains flavonoids, alkaloids, polysaccharides, amino acids, trace elements, vitamins, terpenes and other compounds. Anti-fatigue, low temperature resistance, anti-aging, anti-tumor effects, etc. Its anti-tumor effect is mainly the effect of total flavonoids of Lycium barbarum leaves on the proliferation and apoptosis of human liver cancer cells HepG2, while there are few studies on acidic polysaccharides in Lycium barbarum leaves, especially for Lycium barbarum leaf oligosaccharides among them have not been publicly reported, and how to solve the problem of integrated preparation of wolfberry leaf oligosaccharides and acidic polysaccharides has not been published.

Method used

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  • Preparation method and application of wolfberry leaf oligosaccharide and acidic polysaccharide
  • Preparation method and application of wolfberry leaf oligosaccharide and acidic polysaccharide

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Embodiment 1

[0011] The present invention in concrete implementation, comprises the following steps:

[0012] (1) Take wolfberry leaves, add 8 times the amount of cold water to soak for 2 minutes, filter, dry the dregs at 50°C, and grind them into powder A with a particle size D90 value of 11.5 μm-38.6 μm, and add ultrafine powder A with a weight of 8.5 Soak in twice the amount of ether for 2 hours, filter, evaporate the dregs to make up the ether, add 8 times the weight of superfine powder A to soak in absolute ethanol for 0.5h, reflux at 76°C for 1.1h, filter, evaporate the dregs to make up the ethanol, add superfine powder A to the weight of 8 The volume concentration of double volume is 71% ethanol, reflux extraction at 80°C for 3 hours, filter, evaporate the solvent from the dregs and use it as dregs B for later use. Activated carbon column, AB-8 type macroporous adsorption resin column, Sephadex LH-20 gel column, the eluate was concentrated under reduced pressure into an extract with...

Embodiment 2

[0015] The present invention in concrete implementation, comprises the following steps:

[0016] (1) Take wolfberry leaves, add 15 times the amount of cold water to soak for 2 minutes, filter, dry the dregs at 50°C, and grind them into powder A with a D90 value of 11.5 μm-38.6 μm, and add ultrafine powder A with a weight of 8.5 Soak in twice the amount of ether for 7 hours, filter, evaporate the dregs to make up the ether, add 8 times the weight of superfine powder A to soak in absolute ethanol for 2.5 hours, reflux at 76°C for 2.5 hours, filter, evaporate the dregs to make up the ethanol, add superfine powder A to the weight of 15 The volume concentration of double volume is 82% ethanol, reflux extraction at 80°C for 3 hours, filter, evaporate the solvent from the dregs and use it as dregs B for later use. Activated carbon column, AB-8 type macroporous adsorption resin column, Sephadex LH-20 gel column, the eluate was concentrated under reduced pressure into an extract with a...

Embodiment 3

[0019] The present invention in concrete implementation, comprises the following steps:

[0020] (1) Take wolfberry leaves, add 11 times the amount of cold water to soak for 2 minutes, filter, dry the dregs at 50°C, and grind them into powder A with a particle size D90 value of 11.5 μm-38.6 μm, and add ultrafine powder A with a weight of 8.5 Soak in twice the amount of ether for 4 hours, filter, evaporate the dregs to make up the ether, add 8 times the weight of superfine powder A to soak in absolute ethanol for 1.5 hours, reflux at 76°C for 2.1 hours, filter, evaporate the dregs to make up the ethanol, add superfine powder A to the weight of 11 The volume concentration of double volume is 75% ethanol, reflux extraction at 80°C for 3h, filter, evaporate the solvent from the dregs and use it as dregs B for later use, recover the solvent from the filtrate under reduced pressure, concentrate under reduced pressure at 45°C to obtain an extract with a specific gravity of 1.14, and p...

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Abstract

The invention relates to a preparation method and application of folium lycii oligosaccharides and folium lycii acidic polysaccharides. An ultra-micro pulverization technology is applied to extraction of the folium lycii oligosaccharides and the folium lycii acidic polysaccharides, so that the extraction efficiency of the folium lycii oligosaccharides and the folium lycii acidic polysaccharides and the utilization rate of medicinal materials are greatly improved; integrated efficient preparation of the folium lycii oligosaccharides and the folium lycii acidic polysaccharides is effectively achieved; the folium lycii oligosaccharides and the folium lycii acidic polysaccharides, prepared by the preparation method, can be effectively used for preparing antibacterial medicines or health-care products; the folium lycii acidic polysaccharide I and the folium lycii acidic polysaccharide II are effectively used for preparing anti-tumor medicines.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of wolfberry leaf oligosaccharides and acidic polysaccharides. Background technique [0002] Lycium barbarum leaves are Ningxia Lycium barbarum Lycium barbarum The dry leaves of L., commonly known as Tianjingcao, "Compendium of Materia Medica" claim that it has the effects of "removing annoyance, benefiting will, nourishing five labors and seven injuries, strengthening the heart, removing heat and poison, dispelling sores and swelling, removing wind and improving eyesight". It shows that it mainly contains flavonoids, alkaloids, polysaccharides, amino acids, trace elements, vitamins, terpenes and other compounds. Anti-fatigue, low temperature resistance, anti-aging, anti-tumor effects, etc. Its anti-tumor effect is mainly the effect of total flavonoids of Lycium barbarum leaves on the proliferation and apoptosis of human liver cancer cells HepG2, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H3/04C07H3/06A61P35/00A61P31/04C08B37/00
CPCC07H1/08C07H3/04C07H3/06C08B37/0003
Inventor 刘富岗
Owner HENAN UNIV OF CHINESE MEDICINE