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EMS1/cortactin-targeted multitarget siRNA molecule, and applications thereof

A multi-target and targeted technology, which is applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems that the specific mechanism has not been studied clearly, and solve the problems of poor silencing effect, stability and The effect of good silence and accurate effect

Active Publication Date: 2017-07-04
NANTONG UNIV XINGLIN COLLEGE +2
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Problems solved by technology

There are many literature reports about the close relationship between EMS1 / cortactin and HCC migration and invasion, but the specific mechanism has not been studied clearly

Method used

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  • EMS1/cortactin-targeted multitarget siRNA molecule, and applications thereof
  • EMS1/cortactin-targeted multitarget siRNA molecule, and applications thereof
  • EMS1/cortactin-targeted multitarget siRNA molecule, and applications thereof

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Embodiment Construction

[0034] For convenience, hereinafter, the terms "siRNA", "siRNA sequence" or "siRNA molecule" are interchangeable, and they have the same meaning and scope. Among them, siRNA is a double-stranded structure formed by the annealing of the sense strand and the antisense strand. The siRNA molecule of the present invention is derived from and designed for the functionally conserved region of the open reading frame of the EMS1 / cortactin gene. Various methods can be used to prepare siRNA, such as: chemical synthesis, in vitro transcription, enzyme-cut long-chain dsRNA, vector expression of siRNA, PCR synthesis of siRNA expression elements, etc. The emergence of these methods provides researchers with options. Better access to gene silencing efficiency.

[0035] The present invention adopts chemical synthesis method to prepare siRNA molecule, and this molecule can be used for HCC gene chip detection, EMS1 / cortactin expression to HCC metastases regulation molecular mechanism research, ...

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Abstract

The invention belongs to the technical field of siRNA molecule of targeting tumour-related gene, and relates to an EMS1 / cortactin-targeted multitarget siRNA molecule, and applications thereof. The EMS1 / cortactin-targeted multitarget siRNA molecule comprises following sequences: EMS1 / cortactin siRNA-5 sense strand and antisense strand, EMS1 / cortactin siRNA-6 sense strand and antisense strand, and EMS1 / cortactin siRNA-7 sense strand and antisense strand. Expression of EMS1 / cortactin is inhibited via transfection of human liver cancer (HCC)SMMC-7721 cell line, gene level is about 80%, down-regulating level is relatively stable and accurate. The multitarget siRNA is adopted in HCC gene chip detection, research of EMS1 / cortactin expression on HCC transferring regulating molecular mechanism, and preparation of EMS1 / cortactin-targeted anti-metastasis nucleic acid drugs.

Description

technical field [0001] The invention belongs to the technical field of siRNA molecules targeting tumor-related genes, and relates to multi-target siRNA molecules targeting EMS1 / cortactin and applications thereof. Background technique [0002] Primary liver cancer (HCC) is a complex and heterogeneous tumor with multiple gene changes, and most patients die of postoperative recurrence and distant metastasis. The aggressiveness of HCC cells plays an important role in HCC recurrence and distant metastasis. Studies have shown that there is a layer of 0.2-0.5 micron thick transparent area under the cell membrane, called the cell cortex. The structure of this layer is mainly composed of cross-linked actin microfilaments, which is closely related to important physiological activities such as cytoskeleton reorganization, cell movement, and signal transduction. [1] . The EMS1 gene is located on human chromosome 11q13, and its encoded protein has the ability to bind to actin in the c...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12Q1/68
CPCC12N15/113C12N2310/141C12Q1/6886C12Q2600/178
Inventor 周家名陈莉仲晶飞王桂兰吴美华
Owner NANTONG UNIV XINGLIN COLLEGE
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