A kind of preparation method of cefdinir impurity m
A technology of cefdinir and impurity, which is applied in the field of preparation of cefdinir impurity M, can solve problems such as uneven quality, and achieve the effect of fewer process steps, mild reaction conditions and easy operation
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Embodiment 1
[0026] A preparation method of cefdinir impurity M, comprising the following steps:
[0027] (1) Weigh 10 g of cefdinir raw material, add 20 mL of sulfuric acid, stir and react for 50 h at room temperature at 30° C., to obtain a mixed solution containing cefdinir impurity M;
[0028] (2) Take 1L of the mixed solution containing cefdinir impurity M obtained in step (1), slowly add 700mL of 10% aqueous sodium hydroxide solution, stir to adjust the pH value of the mixed solution to 2.5~3.0, separate with C18 column, and use 1% , 3%, 6%, 9%, 12% acetonitrile solution elution, HPLC detection, collect 9%, 12% acetonitrile elution part, vacuum pump out acetonitrile and then use C18 column to separate, use 3 times column volume 3 times successively %, 6%, 9%, and 12% of acetonitrile aqueous solution to obtain cefdinir impurity M with a yield of 1.5% and a purity of more than 95%.
Embodiment 2
[0030] A preparation method of cefdinir impurity M, comprising the following steps:
[0031] (1) Weigh 10 g of cefdinir raw material, add 35 mL of sulfuric acid, stir and react for 35 h at room temperature at 32° C., to obtain a mixed solution containing cefdinir impurity M;
[0032] (2) Take 1L of the mixed solution containing cefdinir impurity M obtained in step (1), slowly add 700mL of 10% aqueous sodium hydroxide solution, stir to adjust the pH value of the mixed solution to 2.5~3.0, separate with C18 column, and use 1% , 3%, 6%, 9%, 12% acetonitrile solution elution, HPLC detection, collect 9%, 12% acetonitrile elution part, vacuum pump out acetonitrile and then use C18 column to separate, use 3 times column volume 3 times successively %, 6%, 9%, and 12% of acetonitrile aqueous solution to obtain cefdinir impurity M with a yield of 2.3% and a purity of over 95%.
Embodiment 3
[0034] A preparation method of cefdinir impurity M, comprising the following steps:
[0035] (1) Weigh 10 g of cefdinir raw material, add 50 mL of sulfuric acid, and stir at room temperature for 20 h at 35° C. to obtain a mixed solution containing cefdinir impurity M;
[0036] (2) Take 1L of the mixed solution containing cefdinir impurity M obtained in step (1), slowly add 700mL of 10% aqueous sodium hydroxide solution, stir to adjust the pH value of the mixed solution to 2.5~3.0, separate with C18 column, and use 1% , 3%, 6%, 9%, 12% acetonitrile solution elution, HPLC detection, collect 9%, 12% acetonitrile elution part, vacuum pump out acetonitrile and then use C18 column to separate, use 3 times column volume 3 times successively %, 6%, 9%, and 12% of acetonitrile aqueous solution to obtain cefdinir impurity M with a yield of 3.1% and a purity of over 95%.
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