Reagent kit for quickly detecting nucleic acid of canine parvovirus

Abstract

The invention discloses a reagent kit for quickly detecting nucleic acid of canine parvovirus (CPV). The reagent kit comprises lysate, diluent, lyophozyme tubes with primers, detection liquid and test strips. The reagent kit has the advantages that normal-temperature nucleic acid amplification technologies are adopted, different types of enzymatic reaction can be carried out by the aid of DNA (deoxyribonucleic acid) helicase, single-stranded DNA binding proteins, DNA polymerase and the like, and accordingly to-be-detected DNA can be amplified by several millions of times under the condition of the constant temperatures of 37-45 DEG C within 10-30 minutes; the reagent kit is matched with immune colloidal gold chromatographic detection technologies, and accordingly the to-be-detected DNA can be quickly verified; the reagent kit is easy to operate, short in time and quite suitable for quickly diagnosing infectious diseases of pets and has low requirements on instruments.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rapid detection kit for canine parvovirus nucleic acid. Background technique [0002] Canine parvovirus (Canine parvovirus, CPV) is an infectious virus that mainly infects dogs. It was first isolated in 1978 and belongs to the family Parvoviridae and the genus Parvovirus. The disease is transmitted between dogs through direct or indirect contact with their feces. Infectious enteritis and myocarditis caused by canine parvovirus infection in healthy dogs are acute, with high mortality and strong infectivity. It is one of the important infectious diseases of dogs and poses a great threat to the lives of dogs and the dog industry. [0003] Traditional detection of canine parvovirus is mainly based on immunoassays, including immune colloidal gold, enzyme-linked immunosorbent assay, etc., but there are problems such as low specificity and low sensitivity. At present, there are a few de...

AI Technical Summary

Problems solved by technology

However, these technologies also have some limitations. Both PCR technology and real-time fluorescent quantitative PCR technology need to be equipped with high-priced PCR machines or real-time fluorescent quantitative PCR machines, so they cannot be used for grassroots and on-site detection; The technical requirements are high, the reaction temperature is relatively high, easy to cause pollution, and the reaction time is long, so it has not been widely used at present

[0004] For example, Chinese patent CN 101624635 A discloses a canine parvovirus LAMP detection kit and its detection method. The technology requires nucleic acid extraction from the sample. The nucleic acid extraction operation is cumbersome and time-consuming, and has high requirements for operators. ;The reaction temperature of LAMP is 60-65°C, relatively high temperature will easily cause the amplification product to evaporate, resulting in a higher risk of contamination; the reaction solution of the kit needs to be stored at low temperature and transported in cold chain

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