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A kind of selenium-enriched fermented silkworm chrysalis and preparation method thereof

A production method and selenium-enriched technology, which is applied in the field of silkworm chrysalis processing, can solve the problems of lack of selenium-enriched silkworm chrysalis, and achieve the effects of improving quality, enriching nutrition, and enhancing immune function

Active Publication Date: 2020-06-16
ANHUI PROVINCIAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, currently in the technical field of silkworm chrysalis fermentation treatment, there is no technical method for making silkworm chrysalis rich in selenium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] In this embodiment, the bacterial strains of the probiotics conform to the bacterial liquid are constituted as follows:

[0042] Selenium-enriched yeast Candida tropicalis ahas17 3.0×10 9 cfu / ml

[0043] Aspergillus oryzae ahas14 2.0×10 9 cfu / ml

[0044] Bacillus subtilis ahas3 2.0×10 9 cfu / ml

[0045] Lactobacillus fermentum ahas7 1.0×10 9 cfu / ml

[0046] Pediococcus sacidilactici ahas16 1.0×10 9 cfu / ml.

[0047] In this paper, the specific production method is as follows:

[0048] (1) single strain fermentation

[0049] Five strains of yeast Candida tropicalis ahas17, Aspergillus oryzae ahas14, Bacillus subtilis ahas3, Lactobacillus fermentum ahas7, and Pediococcus acidilactici ahas16 were separately fermented to obtain a single-strain fermentation broth. details as follows:

[0050] 1) Preparation of selenium-enriched yeast liquid:

[0051] Candida tropicalis was inoculated in the slant medium and cultured at 28° C. for 32 hours to obtain a slant strain. ...

Embodiment 2

[0071] In this embodiment, the strains of the compound probiotic bacterial liquid constitute:

[0072] Selenium-enriched yeast Candida tropicalis ahas17 4.0×10 9 cfu / ml

[0073]Aspergillus oryzae ahas14 3.0×10 9 cfu / ml

[0074] Bacillus subtilis ahas3 3.0×10 9 cfu / ml

[0075] Lactobacillus fermentum ahas7 2.0×10 9 cfu / ml

[0076] Pediococcus acidilactici ahas16 2.0×10 9 cfu / ml

[0077] In this embodiment, the specific production method is:

[0078] (1) single strain fermentation

[0079] Five strains of yeast Candida tropicalis ahas17, Aspergillus oryzae ahas14, Bacillus subtilis ahas3, Lactobacillus fermentum ahas7, and Pediococcus acidilactici ahas16 were separately fermented to obtain fermentation broth. details as follows:

[0080] 1) Preparation of selenium-enriched yeast liquid:

[0081] Candida tropicalis was inoculated in the slant medium and cultured at 28° C. for 36 hours to obtain the slant strain. Among them, the composition of the slant medium is: glu...

Embodiment 3

[0101] In this embodiment, the strains of the compound probiotic bacterial liquid constitute:

[0102] Selenium-enriched yeast Candida tropicalis ahas17 5.0×10 9 cfu / ml

[0103] Aspergillus oryzae ahas14 4.0×10 9 cfu / ml

[0104] Bacillus subtilis ahas3 4.0×10 9 cfu / ml

[0105] Lactobacillus fermentum ahas7 3.0×10 9 cfu / ml

[0106] Pediococcus acidilactici ahas16 3.0×10 9 cfu / ml.

[0107] In this embodiment, the specific production method is as follows:

[0108] (1) single strain fermentation

[0109] Five strains of yeast Candida tropicalis ahas17, Aspergillus oryzae ahas14, Bacillus subtilis ahas3, Lactobacillus fermentum ahas7, and Pediococcus acidilactici ahas16 were separately fermented to obtain fermentation broth. details as follows:

[0110] 1) Preparation of selenium-enriched yeast liquid:

[0111] Candida tropicalis was inoculated in the slant medium and cultured at 28° C. for 48 hours to obtain the slant strain. Among them, the composition of the slant m...

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Abstract

The invention discloses a selenium-enriched fermented silkworm chrysalis and a preparation method thereof. The present invention first selects and breeds high-selenium-enriched Candida strains, then separately ferments selenium-enriched Candida and five strains of Aspergillus oryzae, Bacillus subtilis, Lactobacillus fermentum, and Pediococcus lactis, and then recombines according to the corresponding ratio. After adding fresh culture medium and protective agent, the compound liquid of selenium-enriched probiotic bacteria is prepared; through the synergy between the probiotic strains, the silkworm chrysalis is fermented by microorganisms to obtain the compound silkworm chrysalis with selenium-enriched and high-protein effects. The composite silkworm chrysalis not only contains a large amount of beneficial microorganisms and metabolites produced during the fermentation process, but also greatly increases the organic selenium content of the highly active silkworm chrysalis, significantly improves the nutritional quality of the silkworm chrysalis, does not need to add selenium additives, and is cheap and high in quality; moreover, Animals can promote the digestion and absorption of nutrients and enhance the immune function of the body after eating, which is of great significance to the feed industry and animal husbandry production.

Description

technical field [0001] The invention belongs to the technical field of silkworm chrysalis treatment, relates to a fermented silkworm chrysalis, in particular to a selenium-enriched fermented silkworm chrysalis and a preparation method thereof. Background technique [0002] Silkworm chrysalis is a by-product of the silk reeling industry. It is rich in nutrients. Its protein content is as high as 50%-70%. The amino acid content is: 3.03% lysine, 0.68% tryptophan, 1.6% methionine, and 3.31% cystine. , balanced ratio, is a high-quality, ideal animal feed protein source. [0003] my country is a big country for raising silkworms, with large output of silkworm chrysalis and abundant resources. However, currently on the market, the processing methods for reeling silkworm chrysalis are very simple and extensive. Most of them are dried and crushed and then added to the feed for use, resulting in poor palatability of the feed and low digestibility of nutrients. [0004] Microbial fe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/20A23K10/12A23K10/18A23K20/20C12N15/01C12N1/16C12N1/20C12N1/14C12R1/74C12R1/69C12R1/125C12R1/225C12R1/01
CPCC12N1/14C12N1/16C12N1/20C12N15/01A23V2400/143A23V2400/231
Inventor 王钰婷李瑞雪汪泰初王伟高新文
Owner ANHUI PROVINCIAL HOSPITAL