Application of KLHL22 gene and expression product thereof to preparation of diagnostic product

A gene expression and product technology, applied in the application field of the preparation of diagnostic products, can solve the problems of high disability rate, affecting the patient's self-care ability and work ability, and irreversible disease.

Inactive Publication Date: 2017-08-04
BEIJING MEDINTELL BIOMED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although in recent years, there has been a lot of progress in the diagnosis and treatment of this disease at home and abroad, but because the disease is a chronic progressive disease with a high disability rate, there is st

Method used

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  • Application of KLHL22 gene and expression product thereof to preparation of diagnostic product
  • Application of KLHL22 gene and expression product thereof to preparation of diagnostic product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Screening of genes differentially expressed in patients with ankylosing spondylitis and normal people

[0032] 1. Sample collection:

[0033] All case samples were collected from outpatients, and the diagnosis of the patients was made by a rheumatologist with rich clinical experience according to the revised New York criteria proposed in 1984, and the diagnosis was confirmed through multiple follow-up visits. Control samples were obtained from age- and sex-matched individuals with no history of arthritis in the Southern Central Sample Bank.

[0034] Peripheral blood samples were randomly collected from 5 patients and 7 normal control individuals on the basis of informed consent.

[0035] 2. Extraction of total RNA from whole blood

[0036] Take 2ml of fasting blood through EDTA.NA 2 For anticoagulation, 0.3ml of whole blood was used to extract RNA using 3S column centrifugal blood total RNA extraction kit (K3620 Shanghai Bocai Biotechnology Co., Ltd.), and ...

Embodiment 2

[0057] Example 2 Large sample verification of differentially expressed genes

[0058] 1. Sample collection

[0059] All case samples were collected from outpatients, and the diagnosis of the patients was made by a rheumatologist with rich clinical experience according to the revised New York criteria proposed in 1984, and the diagnosis was confirmed through multiple follow-up visits. Control samples were obtained from age- and sex-matched individuals with no history of arthritis in the Southern Central Sample Bank.

[0060] Peripheral blood samples were randomly collected from 30 patients and 45 normal controls on the basis of informed consent.

[0061] 2. Extraction of total RNA from whole blood

[0062] Take 2ml of fasting blood through EDTA.NA 2 Anticoagulation, 0.3ml of whole blood was used to extract RNA with 3S column centrifugal blood total RNA extraction kit (K3620 Shanghai Bocai Biotechnology Co., Ltd.), and the RNA extract was stored at -80 degrees.

[0063] 3. A...

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PUM

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Abstract

The invention discloses the application of KLHL22 gene and its expression product in preparing diagnostic products. This diagnostic product can be used for the diagnosis of ankylosing spondylitis. By detecting the level of the KLHL22 gene and its expression products, the risk of the subject suffering from ankylosing spondylitis in the future or the status of ankylosing spondylitis can be determined. The diagnostic product of the present invention takes the peripheral blood of the subject as the detection object, and can realize non-invasive, rapid, sensitive and accurate diagnostic effects.

Description

technical field [0001] The invention belongs to the field of diagnosis and relates to the application of KLHL22 gene and its expression product in the preparation of diagnosis products. Background technique [0002] Ankylosing spondylitis, also known as Von Bechterev's disease or Maritstrumpell's disease, is a chronic, progressive, chronic inflammatory disease involving axial joints. It mainly affects the sacroiliac joints, spinal joints and paravertebral tissues of the pelvis. The main symptoms were low back pain, spinal stiffness and limited range of motion, and X-ray showed sacroilitis on both sides. Because the disease generally invades the sacroiliac joints first, and mainly involves the spine, eventually leading to bony ankylosis of the spine, it is currently called ankylosing spondylitis (AS) at home and abroad. [0003] Ankylosing spondylitis (AS) is an autoimmune disease that mostly occurs in young adults aged 16-40, with a male-to-female ratio of about 4-10:1. T...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
CPCC12Q1/6883C12Q2600/158G01N33/6893G01N2800/101G01N2800/50
Inventor 肖枫李曙光
Owner BEIJING MEDINTELL BIOMED CO LTD
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