Real time polymerase chain reaction (PCR) detection primer, real time PCR probe and kit for detecting duck adenovirus A and duck adenovirus 2
An adenovirus and probe technology, which is applied in DNA/RNA fragments, recombinant DNA technology, and microbial measurement/inspection, to achieve quantitative accuracy, cost savings, and high sensitivity
Inactive Publication Date: 2017-08-15
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology
However, there are currently no research reports on primers and probes that can detect duck adenovirus type A and duck adenovirus type 2 simultaneously at home and abroad. The establishment of the present invention can fill the relevant fields at home and abroad. research gap
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Embodiment 1
[0039] 1. Related test pathogens
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The invention provides a real time polymerase chain reaction (PCR) detection primer, a real time PCR probe and a kit for detecting duck adenovirus A and duck adenovirus 2. The sequences of the primer and the probe are as shown in SEQ ID No. 1-6, and the primer and the probe have very high specificity and sensitivity. At present, the research reports about primers and probes that can detect the duck adenovirus A and the duck adenovirus 2 simultaneously by means of a double-TaqMan real-time fluorescence quantitative PCR detection method are not seen at home and abroad. The establishment of the primer, the probe and the kit appear for the first time in related fields at home and abroad.
Description
technical field [0001] The invention provides duck adenovirus type A and duck adenovirus type 2 real-time fluorescent quantitative PCR detection primers, probes and kits, belonging to the field of veterinary medicine. Background technique [0002] According to the classification of the International Committee on Taxonomy of Viruses, the Adenoviridae family consists of 5 genera, namely the AT-rich adenovirus genus Atadenovirus , Avian Adenovirus Aviadenovirus , fish adenovirus Ichtadenovirus , Mammalian Adenoviruses Mastadenovirus and sialidaseviruses Siadenovirus . Among them, there are 5 species of AT-rich adenovirus, namely bovine adenovirus type D Bovine adenovirus D. Sheep adenovirus type D Ovine adenovirus D. Kangaroo adenovirus type A Possum adenovirus A. Snake adenovirus type A Snake adenovirus A and duck adenovirus type A Duck adenovirus A (referred to as DAdV-A). [0003] The total length of the adenovirus genome is about 33 kd, and the genome i...
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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/701C12Q2600/16C12Q2561/113C12Q2563/107C12Q2545/114C12Q2537/143
Inventor 万春和黄瑜陈翠腾程龙飞傅光华施少华陈红梅傅秋玲刘荣昌
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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