A rotary nucleic acid extraction device and its control method

Abstract

The invention discloses a rotary type nucleic acid extraction device and a control method thereof. The device comprises a multiflux extraction device, a plurality of extraction consumable components, a vertical device which is connected with the multiflux extraction device and a horizontal device which is connected with the extraction consumable components, wherein the multiflux extraction device adopts a rotary mode to mix samples and required extraction reagents uniformly, and the device can provide external force such as magnetic drawing, mixing and heating which is required by nucleic acid extraction and coordinate with a motion in a vertical and / or horizontal direction at the same time, so that the whole process of the nucleic acid extraction is achieved. According to the device, the blending efficiency of the samples and the required extraction reagents is improved, the cross contamination rate between extraction holes is lowered, and the length and the integrity degree of nucleic acid fragments in extraction product are guaranteed.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction and purification, in particular to a rotary nucleic acid extraction device and a control method thereof. Background technique [0002] Nucleic acid is the carrier of genetic information, widely exists in all animal and plant cells and microorganisms, and is one of the most basic substances of life. With the rapid development of molecular biology technology, the research and analysis of nucleic acid has been continuously promoted and applied in clinical diagnosis, food safety, environmental testing, agriculture, forestry and animal husbandry and other fields. [0003] In addition to nucleic acid, biological cells also contain other substances such as proteins and polysaccharides. Therefore, to study and analyze nucleic acid, it is necessary to extract and separate it from the complex biological environment. Therefore, the extraction and purification of nucleic acid is an essential ...

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Problems solved by technology

However, the mixing rate of this method is low, and the mixing effect is poor; at the same time, due to the mixing method of the magnetic bar / stirring sleeve moving up and down, it is easy to generate liquid splashing, resulting in a decrease in the recovery rate of magnetic beads and crossover between the extracted products. Contamination affects the total amount and purity of nucleic acid in the extracted product; in addition, due to the strong vibration of the nucleic acid due to the mixing method of moving up and down, for the extraction of long fragment DNA and single-stranded RNA, the integrity of the nucleic acid fragment in the extracted product cannot be guaranteed Spend

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