Compound probiotic agent for improving immunity of dogs and reducing incidence of diarrhea as well as preparation method and application thereof

A technology of compound probiotics and immunity, applied in the direction of biochemical equipment and methods, applications, medical preparations containing active ingredients, etc., can solve the problems of reducing the incidence of diarrhea and not providing it, so as to reduce the incidence and degree of incidence , increase feed intake, increase the effect of growth rate

Active Publication Date: 2017-08-18
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, in summary, the above patents do not provide a compound probiotic agent that can be widely used in dogs of diffe...

Method used

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  • Compound probiotic agent for improving immunity of dogs and reducing incidence of diarrhea as well as preparation method and application thereof
  • Compound probiotic agent for improving immunity of dogs and reducing incidence of diarrhea as well as preparation method and application thereof
  • Compound probiotic agent for improving immunity of dogs and reducing incidence of diarrhea as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The preparation of four kinds of probiotics of embodiment 1

[0054] Preparation of Lactobacillus plantarum KT-Lp9 inoculum, Lactobacillus casei zhang inoculum, Lactobacillus plantarum P-8 inoculum, Bifidobacterium animalis subsp. lactis V9 inoculum:

[0055] Separately carry out high-density fermentation of 4 strains of fermentation strains: take one ring of activated Lactobacillus plantarum KT-Lp9, Lactobacillus casei zhang, Lactobacillus plantarum P-8, Bifidobacterium animalis subsp. respectively inoculated into MRS medium, cultured at the same temperature of 33-37°C and at the same speed of 50-100rpm for 18-24 hours to obtain the primary seed liquid respectively; %~10% (v / v) was transferred into the MRS medium again for secondary activation, and the secondary seed solution was obtained after the activation time of 18~24 hours; / v) respectively connected to different fermenter culture medium, the temperature is 33-37°C, the rotation speed is 50-100rpm, the ventilati...

Embodiment 2

[0061] Example 2 Preparation of a compound probiotic agent that improves dog immunity and reduces the incidence of diarrhea

[0062] Compound mixing: the Lactobacillus plantarum KT-Lp9 inoculum prepared by Example 1, Lactobacillus casei zhang inoculum, Lactobacillus plantarum P-8 inoculum, Bifidobacterium animalis milk subsp. V9 inoculum in mass ratio 1: 1:1:2 for mixing, and adding diluted carrier skim milk powder for compounding, and the total number of viable bacteria was 2×10 9 CFU / g, 4×10 9 CFU / g and 1×10 10 CFU / g three specifications of compound probiotics;

[0063] The compound probiotic agent is sub-packaged through a powder packaging machine filled with nitrogen gas, 2g / bag.

Embodiment 3

[0064] Example 3 Preparation of a compound probiotic agent that improves dog immunity and reduces the incidence of diarrhea

[0065] Compound mixing: the Lactobacillus plantarum KT-Lp9 inoculum prepared by Example 1, Lactobacillus casei zhang inoculum, Lactobacillus plantarum P-8 inoculum, Bifidobacterium animalis milk subspecies V9 inoculum in mass ratio 4: 4:4:7 for mixing, and adding diluted carrier skim milk powder for compounding, and the total number of viable bacteria was 2×10 9 CFU / g, 4×10 9 CFU / g and 1×10 10 CFU / g three specifications of compound probiotics;

[0066] The compound probiotic agent is sub-packaged through a powder packaging machine filled with nitrogen gas, 2g / bag.

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Abstract

The invention discloses a compound probiotic agent for improving immunity of dogs and reducing incidence of diarrhea as well as a preparation method and application thereof, and belongs to the field of the compound probiotic agent. The compound probiotic agent is prepared by mixing and compounding a lactobacillus plantarum KT-Lp9 microbial agent, a lactobacillus casei zhang microbial agent, a lactobacillus plantarum P-8 microbial agent, a bifidobacterium animalis subsp. lactis V9 microbial agent and a dilution carrier, wherein the quantity of viable bacteria of the four kinds of microbial agents is more than or equal to 2*10<11>CFU/g. The compound probiotic agent with different contents of viable bacteria only can be applied to 96 dogs at different ages for 60 days, so that the immunity of the test dogs can be improved, and the incidence of diarrhea and the sickness degree are reduced. Main representations are that the feed intake and growth rate of the dogs are increased, leukocyte counts, lymphocytes counts and neutrophil counts in blood are controlled, and the contents of four immunologic factors including immune globulin G, interleukin 6, interferon alpha and tumor necrosis factor alpha in the blood can be increased; and meanwhile, the content of secretory immunoglobulin A (SigA) in excrement is increased.

Description

technical field [0001] The invention belongs to the field of compound probiotics, and in particular relates to a compound probiotic for improving dog immunity and reducing the incidence of diarrhea, as well as its preparation and application. Background technique [0002] Diarrhea in dogs is a common clinical disease in dogs. It has many and complicated causes and is difficult to diagnose and treat. Poor, lack of energy, weak constitution. At present, the prevention and treatment of canine diarrhea mainly rely on antibiotics and chemical drugs. Such methods are low-cost and quick-acting in clinical practice, but long-term use will inevitably bring serious threats to public safety, including the increasing number and types of drug-resistant strains , or even bacterial mutation or horizontal transfer of drug-resistant genes, destroying the intestinal micro-ecological system of the body, making the body more sensitive to new pathogens, thus making the treatment of diarrheal di...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61K35/747A61P1/12A23K10/18C12R1/245C12R1/25C12R1/01
CPCA61K35/745A61K35/747C12N1/20
Inventor 黄卫强张永军
Owner INNER MONGOLIA SCI PLUS BIOTECH
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