Concentrating Particles in Microfluidic Devices

A technology of microfluidic devices and microfluidic channels, which can be used in individual particle analysis, measurement devices, fluid controllers, etc., to solve problems such as limitations and low yields

Active Publication Date: 2020-10-02
THE GENERAL HOSPITAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, typical microscopic sorting and filtration devices may be limited in the amount of fluid they can process in a given period of time (i.e., low throughput), potentially putting such devices at a disadvantage relative to their macroscopic counterparts

Method used

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  • Concentrating Particles in Microfluidic Devices
  • Concentrating Particles in Microfluidic Devices
  • Concentrating Particles in Microfluidic Devices

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0240] Example 1: Cell-free layer growth and percent siphoning

[0241] The combined siphon and inertial focusing design has the advantage of fast acting inertial forces that create a particle-free layer near the walls of the microfluidic channel. This layer of particle-free fluid is then controllably siphoned away, again leaving the particles closer to the wall where the inertial forces are strongest. The process of agglomeration and siphoning can be repeated until the desired volume reduction is obtained. When using microfluidic devices to enhance the concentration of particles within a fluid or to extract a particle-free fluid, important design considerations may include controlling the percentage of fluid that is siphoned relative to the kinetics of the formation of a particle-free layer. In inertial aggregation systems, aggregation behavior is the cumulative result of many parameters including channel geometry as well as flow velocity (see, for example, Di Carlo, D. "I...

example 2

[0248] Example 2: Flow rate dependence

[0249] Another factor that may be considered in microfluidic systems for performing volume reduction and / or increasing particle concentration within a fluid is the flow rate of a fluid sample through the microfluidic device. Therefore, the sensitivity to flow rate was also investigated. The yields of both the 10x and 50x devices were analyzed between input flow rates of 100 μl / min to 1000 μl / min using isolated white blood cells (buffy coat). Yield is calculated on a relative basis between the number of cells in the stream flowing in the aggregation channel and the number of cells in the second fluid flow region, or alternatively, as the total number of cells in the stream flowing in the aggregation channel. Divide the number by the total cells in the combination of aggregation channels and second fluid flow channels. The high throughput of the device, greater than 95%, was maintained between 400 and 600 μl / min, but beyond this range...

example 3

[0252] Example 3: Size Dependency

[0253]The inertial force depends largely on the size of the particles being aggregated. Therefore, the performance of the combined inertial aggregation and siphon device was evaluated for sensitivity to particle size. Specifically, various polystyrene particle sizes (4 μm-10 μm) were run simultaneously through the 10x and 50x setups in order to determine the size range of particles deflected from the aggregation channel to the second fluid flow region where the "particle free" layer is desired. Figure 13 is a plot of the previous experiment and shows that smaller particle sizes have lower relative yields than larger particle sizes (i.e., (total cells in product) / (total cells in product+total cells in waste )), that is, the smaller the particle, the more likely it is that the particle will escape the aggregation channel through the gaps between the island structures. If a relative yield above 90% is desired, the cutoff particle size for ...

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Abstract

A microfluidic device comprising: a first microfluidic channel; a second microfluidic channel extending along the first microfluidic channel; and an island separating the first microfluidic channel from the second microfluidic channel wherein each island is separated from an adjacent island in the array by an opening fluidly coupling the first microfluidic channel to the second microfluidic channel, wherein the first microfluidic channel , the second microfluidic channel and the island are arranged such that the fluidic resistance of the first microfluidic channel increases relative to the fluidic resistance of the second microfluidic channel along the longitudinal direction of the first microfluidic channel , such that during use of the microfluidic device, a portion of the fluid sample flowing through the first microfluidic channel passes through one or more of the openings between adjacent islands to the second microfluidic channel.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Application No. 62 / 074,213, filed November 3, 2014, and U.S. Provisional Application No. 62 / 074,315, filed November 3, 2014, each of which is hereby incorporated by reference in its entirety . technical field [0003] The present disclosure relates to the concentration of particles in microfluidic devices. Background technique [0004] Particle separation and filtration has been widely used in various industries and fields. Examples of these applications include chemical process and fermentation filtration, water purification / wastewater treatment, sorting and filtering blood components, concentrating colloidal solutions, and purifying and concentrating environmental samples. Various macroscopic techniques have been developed for these applications, including methods such as centrifugation and filter-based techniques. Typically, such techniques require large, bu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14
CPCG01N15/1484B01L3/502715B01L3/502753B01L3/502761G01N1/4077B01L2200/0647B01L2200/0631B01L2200/0668B01L2200/0652B01L2300/0877G01N2001/4088G01N2015/149G01N2015/1493G01N2015/1486B01L3/502746B01L3/502776B01L2400/043B01L2400/0457B01L2400/0487B01L2400/082B01L2200/0694B01L2300/0858B01L2300/0883B01L2300/0887B01L2400/086G01N2015/0053G01N15/0255B01L2200/0684B01L2300/0681B01L2300/185B01L2400/0409B01L2400/0415B01L2400/0436A61K35/28B01L2200/12G01N15/0618G01N2015/0065G01N2015/0288
Inventor R·卡普尔K·C·史密斯M·托纳
Owner THE GENERAL HOSPITAL CORP
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