Preparation method of effective part of Inula helenium L. sesquiterpene lactone and application thereof to pancreatic cancer resistance

A technology of effective parts and terpene lactones is applied in the preparation of effective parts of Inulin sesquiterpene lactones, and the application field of anti-pancreatic cancer can solve the problem that the inhibitory activity of human pancreatic cancer cells is rarely reported in literature, etc. The effect of good pharmacological activity, strong elution ability and high purity

Inactive Publication Date: 2017-09-01
HANGZHOU FIRST PEOPLES HOSPITAL
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the treatment of cancer, but it is mainly leukemia, and many literature reports mainly focus on: the growth inhibitory activity of a sesquiterpene lactone monomer of inulin against human tumor cells in vitro, and the specific effective substances of inulin The effective part of sesquiterpene lactone, the inhibitory activity of the difficult-to-treat human pancreatic cancer cells is rarely reported in the literature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of effective part of Inula helenium L. sesquiterpene lactone and application thereof to pancreatic cancer resistance
  • Preparation method of effective part of Inula helenium L. sesquiterpene lactone and application thereof to pancreatic cancer resistance
  • Preparation method of effective part of Inula helenium L. sesquiterpene lactone and application thereof to pancreatic cancer resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of the effective part of local woody sesquiterpene lactone comprises the following steps:

[0032] A. Extraction: Powder the medicinal material of inulin, pass through a 40-mesh sieve for later use. Take 10 kg of crude powder of inula chinensis medicinal material, add an appropriate amount of 95% ethanol to cold soak for 24 hours, then extract by percolation, and collect the percolation liquid. The volume of the total solvent for extraction is equivalent to 15 times of the quality of the medicinal material (150 L of 95% ethanol is used in total).

[0033] B. Extraction: Concentrate the percolation liquid obtained in step A under reduced pressure until it has no alcohol smell, add water to suspend to 2L, extract with 2L of ethyl acetate and 2L of n-butanol, extract three times with each extraction solvent, and combine each time The extracts of inulin were obtained from ethyl acetate extract and n-butanol extract respectively. Through in vitro anti-p...

Embodiment 2

[0038] A. Extraction: Take 10 kg of Elecampa officinalis medicinal material, add 10 times the amount (100 L) of 95% ethanol, reflux extraction, each extraction time is 2 hours, extract 2 times in total, and combine the two extracts. A total of 200L of 95% ethanol was used.

[0039] B. Extraction: Concentrate the extract obtained in step A under reduced pressure until it has no alcohol smell, add water to suspend to 2L, extract with 2L of ethyl acetate and 2L of n-butanol respectively, extract three times with each extraction solvent, and combine each time The extracts of inulin were obtained from ethyl acetate extract and n-butanol extract respectively. Through in vitro anti-pancreatic cancer activity tracking, the effective components of inula sesquiterpene lactone were mainly concentrated in ethyl acetate extract, and ethyl acetate The solvent was recovered from the extract under reduced pressure to obtain 397.4 g of inulin ethyl acetate extract.

[0040] C. Column chromato...

Embodiment 3

[0043] A. Extraction: Powder the medicinal material of inulin, pass through a 40-mesh sieve for later use. Take 10kg of the coarse powder of the medicinal material of inulin, add an appropriate amount of 95% ethanol for cold soaking for 24 hours, and then extract by percolation. The volume of the extraction solvent is equivalent to 15 times the mass of the medicinal material (150L of 95% ethanol is used in total), and the percolation solution is collected.

[0044] B. Freeze-drying: the percolation solution obtained in step A was concentrated under reduced pressure until it had no alcohol smell, and the thick extract was dried by freeze-drying to obtain 468.4 g of freeze-dried powder.

[0045] C. Column chromatography: Take about 200 g of the freeze-dried powder of the above-mentioned inulin extract, put it on a 100-200-mesh normal-phase silica gel column for separation, and then use a plurality of different solvent gradients for elution, and each elution solvent is made of pet...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a preparation method of an effective part of asteraceae Inula helenium L. sesquiterpene lactone. The preparation method comprises the following steps: A, performing percolation extraction on Inula helenium L. with an extraction solvent to obtain an extract; B, concentrating the extract, adding water for suspending, extracting with ethyl acetate, and concentrating an extract to obtain an Inula helenium L. ethyl acetate extract; C, performing normal phase column chromatography on the Inula helenium L. ethyl acetate extract, and eluting with an eluent; and performing in-vitro anti-pancreatic cancer activity tracing in combination with high-performance liquid chromatography, collecting an eluate, and drying to obtain the effective part of the Inula helenium L. sesquiterpene lactone, wherein the eluent at least comprises petroleum ether and ethyl acetate according to a volume ratio of (20-16): 1. The effective part of the Inula helenium L. sesquiterpene lactone, provided by the preparation method, is strong in bioactivity, simple in operation and low in production cost, has the advantages of stable and controllable quality and the like, and can be applied to preparation of medicines for treating the pancreatic cancer.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a preparation method of effective parts of inulin sesquiterpene lactone and its application in anti-pancreatic cancer. Background technique [0002] There are more than 100 species of Inula L. in the family Asteraceae, mainly distributed in Europe, Africa and Asia, mainly in the Mediterranean region. There are more than 20 species and many varieties of Inula in my country, some of which are widely distributed; the endemic species in my country are mainly concentrated in the west and southwest. Several species of Inula genus are often used medicinally. [0003] Inula helenium L. is a plant of the genus Inula inulaceae. It has been used for many years in my country and is now included in the 2015 Chinese Pharmacopoeia. Tumuxiang, pungent, bitter, warm, returns to the liver and spleen meridian, has the effects of invigorating the spleen and stomach, promoti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61K36/28C07D307/92A61P35/00
CPCA61K31/365A61K36/28C07D307/92A61K2300/00
Inventor 林能明张博阎优优
Owner HANGZHOU FIRST PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap