Preparation method of effective part of Inula helenium L. sesquiterpene lactone and application thereof to pancreatic cancer resistance
A technology of effective parts and terpene lactones is applied in the preparation of effective parts of Inulin sesquiterpene lactones, and the application field of anti-pancreatic cancer can solve the problem that the inhibitory activity of human pancreatic cancer cells is rarely reported in literature, etc. The effect of good pharmacological activity, strong elution ability and high purity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] The preparation method of the effective part of local woody sesquiterpene lactone comprises the following steps:
[0032] A. Extraction: Powder the medicinal material of inulin, pass through a 40-mesh sieve for later use. Take 10 kg of crude powder of inula chinensis medicinal material, add an appropriate amount of 95% ethanol to cold soak for 24 hours, then extract by percolation, and collect the percolation liquid. The volume of the total solvent for extraction is equivalent to 15 times of the quality of the medicinal material (150 L of 95% ethanol is used in total).
[0033] B. Extraction: Concentrate the percolation liquid obtained in step A under reduced pressure until it has no alcohol smell, add water to suspend to 2L, extract with 2L of ethyl acetate and 2L of n-butanol, extract three times with each extraction solvent, and combine each time The extracts of inulin were obtained from ethyl acetate extract and n-butanol extract respectively. Through in vitro anti-p...
Embodiment 2
[0038] A. Extraction: Take 10 kg of Elecampa officinalis medicinal material, add 10 times the amount (100 L) of 95% ethanol, reflux extraction, each extraction time is 2 hours, extract 2 times in total, and combine the two extracts. A total of 200L of 95% ethanol was used.
[0039] B. Extraction: Concentrate the extract obtained in step A under reduced pressure until it has no alcohol smell, add water to suspend to 2L, extract with 2L of ethyl acetate and 2L of n-butanol respectively, extract three times with each extraction solvent, and combine each time The extracts of inulin were obtained from ethyl acetate extract and n-butanol extract respectively. Through in vitro anti-pancreatic cancer activity tracking, the effective components of inula sesquiterpene lactone were mainly concentrated in ethyl acetate extract, and ethyl acetate The solvent was recovered from the extract under reduced pressure to obtain 397.4 g of inulin ethyl acetate extract.
[0040] C. Column chromato...
Embodiment 3
[0043] A. Extraction: Powder the medicinal material of inulin, pass through a 40-mesh sieve for later use. Take 10kg of the coarse powder of the medicinal material of inulin, add an appropriate amount of 95% ethanol for cold soaking for 24 hours, and then extract by percolation. The volume of the extraction solvent is equivalent to 15 times the mass of the medicinal material (150L of 95% ethanol is used in total), and the percolation solution is collected.
[0044] B. Freeze-drying: the percolation solution obtained in step A was concentrated under reduced pressure until it had no alcohol smell, and the thick extract was dried by freeze-drying to obtain 468.4 g of freeze-dried powder.
[0045] C. Column chromatography: Take about 200 g of the freeze-dried powder of the above-mentioned inulin extract, put it on a 100-200-mesh normal-phase silica gel column for separation, and then use a plurality of different solvent gradients for elution, and each elution solvent is made of pet...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com