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Polypyrrole bioconductive hydrogel and its preparation method and application

A conductive hydrogel and polypyrrole technology, applied in the field of biomedical materials, can solve the problems of unreleased large-volume drugs and unsatisfactory loading of neutral drugs, etc., achieve an excellent extracellular matrix environment, increase drug loading rate, and guide nerves Effects of axon outgrowth

Active Publication Date: 2019-11-26
深圳南泥湾科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The system can achieve a higher drug loading level, and can solve the problems of unsatisfactory loading of neutral drugs by simple CPs and the inability to release large-volume drugs from the CPs system.

Method used

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  • Polypyrrole bioconductive hydrogel and its preparation method and application
  • Polypyrrole bioconductive hydrogel and its preparation method and application
  • Polypyrrole bioconductive hydrogel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Preparation of polymer hydrogel

[0029] A homogeneous EDC / NHS catalytic system was used to prepare hydrogels by cross-linking carboxymethyl chitosan. Add 0.1g CMCS to a 250mL three-neck flask, then add 30mL deionized water, stir magnetically for 30-60min, and dissolve. Add 0.01g EDC and 0.006g NHS into 40mL dimethyl sulfoxide (DMSO), and dissolve with magnetic stirring. The DMSO solution was transferred to the dropping funnel, and added dropwise into the carboxymethyl chitosan aqueous solution, the reaction temperature was 25° C., and the reaction time was 15 hours. The reaction equation of the cross-linking process of carboxymethyl chitosan is:

[0030]

[0031] The product was dialyzed for 48h, and freeze-dried for 24h to obtain a carboxymethyl chitosan hydrogel freeze-dried product; figure 1 (b) Electron micrograph of the carboxymethyl chitosan hydrogel. The pore size of the hydrogel was measured to be 80-200 μm.

[0032] 2) Preparation of polypyrrole

[...

Embodiment 2

[0042] 1) Preparation of polymer hydrogel

[0043] Take carboxymethyl chitosan (CMCS) as an example. A homogeneous EDC / NHS catalytic system was used to prepare hydrogels by cross-linking carboxymethyl chitosan. Add 0.2g CMCS to a 250mL three-neck flask, then add 30mL deionized water, stir magnetically for 30-60min, and dissolve. Add 0.02g EDC and 0.012g NHS into 60mL dimethyl sulfoxide (DMSO), and dissolve with magnetic stirring. The DMSO solution was transferred to the dropping funnel, and added dropwise into the carboxymethyl chitosan aqueous solution, the reaction temperature was 25° C., and the reaction time was 15 hours. The product was dialyzed for 48 hours and freeze-dried for 24 hours to obtain a hydrogel freeze-dried product;

[0044] 2) Preparation of polypyrrole

[0045] A three-electrode system was adopted, with ITO conductive glass as the working electrode, platinum wire as the auxiliary electrode, a saturated calomel electrode as the reference electrode, and ...

Embodiment 3

[0049] 1) Preparation of polymer hydrogel

[0050] Take carboxymethyl chitosan (CMCS) as an example. A homogeneous EDC / NHS catalytic system was used to prepare hydrogels by cross-linking carboxymethyl chitosan. Add 0.3g CMCS to a 250mL three-neck flask, then add 50mL deionized water, stir magnetically for 30-60min, and dissolve. Add 0.03g EDC and 0.018mg NHS into 60mL dimethyl sulfoxide (DMSO), and dissolve with magnetic stirring. The DMSO solution was transferred to the dropping funnel, and added dropwise into the carboxymethyl chitosan aqueous solution, the reaction temperature was 25° C., and the reaction time was 15 hours. The product was dialyzed for 48 hours and freeze-dried for 24 hours to obtain a hydrogel freeze-dried product;

[0051] 2) Preparation of polypyrrole

[0052] A three-electrode system was adopted, with ITO conductive glass as the working electrode, platinum wire as the auxiliary electrode, a saturated calomel electrode as the reference electrode, and...

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Abstract

The invention relates to polypyrrole biological conductive hydrogel, as well as a preparation method and application thereof. The preparation method comprises the following steps; preparing carboxymethylchitosan hydrogel from carboxymethyl chitosan by using a crosslinking agent under a certain condition, and performing freeze-drying treatment; performing electrochemical synthesis on a pyrrole monomer to obtain polypyrrole, adding a dopant into the polypyrrole to perform modification and performing ball-milling and crushing to enable the particle size to be matched with the pore diameter of pores of the carboxymethylchitosan hydrogel; blending nerve growth factors into water, adding modified polypyrrole particles, stirring and dispersing uniformly, putting the carboxymethylchitosan hydrogel to enable the solution to be completely absorbed, adding water, vibrating, and completely swelling and balancing to obtain the polypyrrole biological conductive hydrogel. When the polypyrrole biological conductive hydrogel is applied to neural restoration, sustained release of neurotrophic factors can be completed, nerve cells can be subjected to certain electrical stimulation through bioelectrical or external electrical stimulation, proliferation and differentiation of the nerve cells are promoted through double effects of electric stimulation and neurotrophic factor induction, and nerve axon growth is guided.

Description

technical field [0001] The invention relates to a polypyrrole bioconductive hydrogel and a preparation method and application thereof, belonging to the field of biomedical materials. Background technique [0002] Peripheral nerve injury is very common in clinical practice. So far, nerve anastomosis, autologous nerve transplantation, allogeneic nerve transplantation, autologous non-nerve tissue transplantation, and biomaterial replacement have been performed. However, several treatment methods have their own advantages and disadvantages. The problem of nerve defect has not been fundamentally solved. Studies have shown that nerve growth factor (NGF) has neurotrophic and neuroprotective effects, can promote the repair of nerve damage, induce the directional growth of nerve fibers, stimulate the development of cell bodies and dendrites, and promote the differentiation and development of neurons. At the same time, a large number of experiments have confirmed that electrical stim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/18A61L27/52A61K9/06A61K47/36A61K47/34C08J3/075C08J3/24C08G73/06C08L5/08
CPCA61K9/0019A61K9/06A61K47/34A61K47/36A61L27/18A61L27/20A61L27/52A61L2300/414A61L2430/32C08G73/0611C08J3/075C08J3/24C08J2305/08C08L5/08
Inventor 徐海星布颖许沛虎黄志军郭兴蕾张希马慧向梅闫秀美包昀
Owner 深圳南泥湾科技有限公司
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