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A method suitable for rapid propagation of various types of plants
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A plant and fast technology, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of increasing manpower and material resources, different tissue culture and reproduction methods, and affecting the cost of factory production, so as to reduce manpower and Material resources, the effect of reducing production costs
Inactive Publication Date: 2019-06-07
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI +1
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Problems solved by technology
In each tissue culture research center, the research object will not be a single species, usually two or more main research products, but the suitable explants and tissue culture propagation methods for each plant are different, and it takes a lot of money time and energy to explore efficient and stable tissue culture propagation methods for each plant, which greatly increases the input of manpower and material resources and directly affects the cost of factory production
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Embodiment 1
[0027] Example 1, Tissue Culture Rapid Propagation of Honeysuckle, Houttuynia cordata, Yaerqin, Shuangjiulixiang
[0028] 1. Culture medium preparation and sterilization
[0030] Proliferation medium: ER+KT2.0mg / L+paclobutrazol1.0mg / L+3% sucrose+0.75% agar. The above medium was sterilized at 121°C for 20 minutes.
[0031] 2. Primary culture
[0032]In step (1) of the above method, the stem section of the annual branch of honeysuckle is selected as the explant for cultivation, and the stem section is cut into 1.0-2.0 cm for later use; after the rhizome of Houttuynia cordata is first cleaned, it is placed in clean water and cultivated for about 10 days, and then the Cut the rhizomes into 1.0-2.0 cm and set aside; select smaller plants for duck celery, remove the roots, petioles and leaves, keep the base at a height of about 2 cm, and peel off several layers of bracts for later use; peel off four numbers ...
Embodiment 2
[0038] Example 2, Tissue Culture Rapid Propagation of Honeysuckle, Houttuynia cordata, Yaerqian, Shuangjiulixiang
[0039] 1. Culture medium preparation and sterilization
[0041] Proliferation medium: ER+KT2.5mg / L+paclobutrazol 1.5mg / L+3% sucrose+0.75% agar. The above medium was sterilized at 121°C for 20 minutes.
[0042] Different explants of honeysuckle, Houttuynia cordata, Yaerqin, and Sishu Jiulixiang were taken from tissue culture to rapidly propagate different explants, and the basic operation method was the same as that of Example 1; the effect was not much different from that of Example 1, except that the stem base of Sishu Jiulixiang Partialized more brown matter, but does not affect its growth.
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Abstract
The invention discloses a method applicable to rapid propagation of various plants. The method comprises the following steps: (1) selecting explants: selecting tender stem segments of honeysuckle, rhizomes of houttuynia cordata, top buds of cryptotaenia japonica and seeds of Murraya tetramera Huang as the explants, disinfecting and culturing; (2) performing primary culture: inoculating a solid culture medium containing ER, 0.6-2.5mg / L of 6-BA, and 0.2-1.5mg / L of KT, as well as added sucrose and agar with the disinfected stem segments, rhizomes, top buds or seeds obtained in the step (1), culturing for 20-30 days, and inducing budding; (3) performing propagation subculture: inoculating a solid culture medium containing ER, 0.01-3.0mg / L of KT, and 0.2-3.0mg / L of paclobutrazol, as well as added sucrose and agar with sterile seedlings or stem segments obtained in the following step (2), and culturing for 30 days to obtain buds and roots at the same time. With the method, large quantities of high-quality seedlings of various plants are timely provided for scale development.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture and relates to a core technology suitable for rapid propagation of various types of plants. Background technique [0002] Honeysuckle (Lonicerajaponica Thund.), also known as honeysuckle, double flower, silver flower, Erbao flower, is a perennial semi-evergreen vine of Lonicera genus Lonicera. Honeysuckle has the effects of clearing heat and detoxification, reducing inflammation and swelling, and delaying aging. It can effectively prevent viruses such as "SARS", bird flu, and H1N1 influenza, and also has anti-cancer and anti-AIDS effects. In addition, honeysuckle also contains a variety of antioxidant active ingredients, which can lower blood lipids and be used for the prevention and treatment of cardiovascular and cerebrovascular diseases. With the development of the green revolution, human beings are gradually returning to nature. Natural medicines are more and more concerned and v...
Claims
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Application Information
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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄丽芳夏新界
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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