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Wheat spike density QTL linked HRM (high resolution melting) molecular marker and application thereof

A molecular marker, wheat technology, applied in the fields of molecular biology and crop genetics and breeding, can solve the problem that the research on ear density is still less

Active Publication Date: 2017-09-19
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Previously detected QTL loci controlling panicle density on wheat 1A, 1B, 2A, 2D, 3D, 4A, 4D, 5A, 5B, 5D, 6A, 7A, 7B and 7D, but compared with panicle length, The number of grains per ear and the total number of spikelets, there are still few studies on ear density

Method used

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  • Wheat spike density QTL linked HRM (high resolution melting) molecular marker and application thereof
  • Wheat spike density QTL linked HRM (high resolution melting) molecular marker and application thereof
  • Wheat spike density QTL linked HRM (high resolution melting) molecular marker and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1 Acquisition of wheat spikelet number QSd.sau-7A and its molecular marker 0C32-715

[0033] 1. The female parent of Tibetan semi-wild wheat Q1028 was crossed with the wheat variety Zhengmai 9023 as the male parent to obtain the hybrid F1. F1 was self-crossed to obtain F2, and 186 individual plants were randomly selected in F2 for self-crossing. Harvest a single plant, continue selfing in the next year, and repeat this until the F8 generation, and obtain recombinant inbred lines containing 186 lines to form a genetic mapping population.

[0034] 2. Phenotypic identification of panicle density characteristics of recombinant inbred lines

[0035] Phenotypic identification of panicle length and spikelet number of recombinant inbred lines at wheat maturity stage.

[0036] 3. DNA extraction: The DNA of parent Q1028, Zhengmai 9023 and recombinant inbred lines population plants was extracted by CTAB method.

[0037] 4. Use comparative genomics methods to compare with...

Embodiment 2

[0044] Example 2 Application of Molecular Marker OC32-715 in Identifying Ear Density QTLQSd.sau-7A Wheat Plants

[0045] 1. The offspring QZ2 in the RIL population of (Q1028×Zhengmai 9023) was used as the female parent, and the common wheat line Zhengmai 9023 was used as the male parent to construct recombinant inbred lines, and 8 lines were randomly selected from the offspring lines.

[0046] 2. Perform OC32-715 marker detection on the obtained 8 strains, the specific method is: extract the DNA of the 8 strains at the seedling stage; use it as a template to perform fluorescent quantitative PCR on the molecular marker 0C32-715.

[0047] PCR amplification system: 5 μL of SsoFast EvaGreen supermix, 300 ng of upstream primer and 300 ng of downstream primer, 100 ng of template DNA, Dnaes / RNase-free deionized water to a total of 10 μL.

[0048] Fluorescent quantitative PCR amplification program: pre-denaturation at 95°C for 5 minutes; denaturation at 94°C for 15 s, annealing at 59....

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Abstract

The invention discloses a wheat spike density QTL linked HRM (high resolution melting) molecular marker and application thereof and provides a wheat spike density QTL Qsd.sau-7A linked molecular marker. The molecular marker is 0C32-715, and a nucleotide sequence is shown as SEQ ID NO:1. According to detection and analysis, the molecular marker is capable of closely tracking wheat spike density QTL to predict spikelet dense insertion characteristics, and then selecting and breeding of a high-spike-density variety (strain) can be realized through molecular assisted selection. The invention further provides application of the molecular marker 0C32-715 to wheat breeding. By adoption of the method, accuracy in wheat spike density prediction can be improved in a certain range to make it convenient for quickly screening out the wheat variety or strain with spike density value increasing QTL for breeding, and accordingly a wheat high-yield variety selection process can be greatly accelerated.

Description

technical field [0001] The present invention relates to molecular biology and crop genetic breeding, in particular to molecular markers related to wheat ear density traits, in particular to HRM markers linked to wheat ear density QTL loci Qsd.sau-7A, and the markers Applications. Background technique [0002] Wheat (Triticum aestivum L.) is one of the most important food crops in the world. At present, the world's arable land area is decreasing and the population is increasing. It is urgently expected that the annual output of wheat will increase rapidly. The three factors that constitute the yield of wheat are the number of grains per ear, the number of ears per mu and the weight of grains. Spike traits determine the number of spikelets, which are related to yield to a certain extent. On the other hand, panicle morphology is also meaningful for wheat species identification. Factors affecting the number of grains per spike in wheat include spike length, number of spikelet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 马建兰秀锦孙敏郑有良魏育明刘亚西陈国跃江千涛祁鹏飞
Owner SICHUAN AGRI UNIV
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