Monoclonal antibody for anti-HPV (Human Papillomavirus) 16 type E7 protein, test paper and detection kit

A monoclonal antibody and detection kit technology, which is applied in the field of detection test strips, detection kits, and monoclonal antibodies, can solve problems such as difficult promotion, high cost, and complicated operating procedures, and achieve the effect of convenient treatment

Inactive Publication Date: 2017-09-22
ZHUHAI MEIHUA MEDICAL TECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is a common limitation: the operation procedure is complex, profes

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  • Monoclonal antibody for anti-HPV (Human Papillomavirus) 16 type E7 protein, test paper and detection kit
  • Monoclonal antibody for anti-HPV (Human Papillomavirus) 16 type E7 protein, test paper and detection kit
  • Monoclonal antibody for anti-HPV (Human Papillomavirus) 16 type E7 protein, test paper and detection kit

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Embodiment Construction

[0028] The present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0029] The embodiment of the present application provides a monoclonal antibody against HPV16 type E7 protein, which has the following binding sites with HPV16 type E7 protein: 30-36 amino acids of HPV16 type E7 protein, 36-43 amino acids of HPV16 type E7 protein, HPV16 The 62-73 amino acid of type E7 protein or the 73-77 amino acid of HPV16 type E7 protein, the antibody specifically binds to the HPV16 type E7 protein.

[0030] The embodiment of the present application provides a detection test paper of HPV16 type E7 protein, such as figure 1As shown, it includes a backing 1 and a sample pad 2 fixed on the backing 1 , a bonding pad 3 , an NC membrane 4 and an absorbent pad 5 . Among them, the sample pad 2 is used to place the sample to be tested, the binding pad 3 is attached and fixed under the sample pad 1, and the NC membrane ...

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Abstract

A monoclonal antibody against HPV16 type E7 protein, a detection test paper and a detection kit. The binding site of monoclonal antibody against HPV16 E7 protein is: amino acid 30-36 of HPV16 E7 protein, amino acid 36-43 of HPV16 E7 protein, amino acid 62-73 of HPV16 E7 protein or HPV16 Amino acids 73-77 of type E7 protein. The detection test paper includes a backing and a sample pad fixed on the backing, a binding pad, an NC film and an absorbent pad; the binding pad is attached and fixed under the sample pad, one end of the NC film is attached and fixed under the binding pad, and the other end Link to each other with water-absorbing pad; NC membrane is sprayed with detection antibody, and detection antibody comprises the monoclonal antibody of the anti-HPV16 type E7 protein of claim 1 and goat anti-mouse secondary antibody, the monoclonal antibody of anti-HPV16 type E7 protein is used as detection line, Goat anti-mouse secondary antibody is used as a quality control line; the binding pad is coated with fluorescent microsphere-labeled antibody. The application can detect HPV type E7 protein simply and quickly.

Description

technical field [0001] The application relates to the field of antibodies, in particular to a monoclonal antibody against HPV16 type E7 protein, a detection test paper and a detection kit. Background technique [0002] Cervical cancer is a cancer with a very high incidence in women and is associated with high-risk human papillomavirus infection in 99.7% of cases. There are about 520,000 cases of cervical cancer and nearly 260,000 deaths worldwide each year. There are more than 100 different isolates of HPV that have been broadly subdivided into high-risk and low-risk subtypes based on their association with cervical cancer or with benign cervical lesions or atypical hyperplasia. Low-risk HPVs include HPV6, 11, 42, 43, 44, etc., which often cause external genital warts and other benign lesions, including low-grade cervical intraepithelial lesions (CIN I), and high-risk HPVs include HPV16, 18, 31, 33, 35 , 39, 45, 51, 52, 56, 58, 59, 68, CP8304 and other subtypes, which are r...

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Application Information

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IPC IPC(8): G01N33/68G01N33/558G01N33/577G01N33/574G01N33/569G01N33/52G01N33/533G01N21/64
CPCG01N33/68G01N21/6428G01N33/52G01N33/533G01N33/558G01N33/56983G01N33/57411G01N33/5748G01N33/577G01N2021/6439G01N2333/025G01N2800/36
Inventor 乌月恒
Owner ZHUHAI MEIHUA MEDICAL TECH LTD
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