Reagent card for rapidly detecting IgG subtypes and preparation method thereof

Abstract

The invention relates to a reagent card for rapidly detecting IgG subtypes and a preparation method thereof. The reagent card comprises a vertical fixing plate (1), wherein a horizontal supporting plate (2) is arranged at the top of the vertical fixing plate (1); eight microcolumn-type gel tubes (3) are arranged on the horizontal supporting plate (2); each microcolumn-type gel tube (3) comprises a reaction chamber (3.1) and a gel separating column (3.2); a circle of flange (3.3) is arranged at the top of the reaction chamber (3.1); multiple layers of separating screens (3.4) are arranged inside the gel separating column (3.2) from top to bottom; supporting frames (4) are arranged below the horizontal supporting plate (2); the microcolumn-type gel tubes (3) are inserted into the supporting frames (4); protective films (5) are arranged above the horizontal supporting plate (2); the protective films (5) cover the flanges (3.3) of the eight microcolumn-type gel tubes (3). According to the reagent card for rapidly detecting the IgG subtypes and the preparation method thereof, the reagent card is high in sensitivity, good in specificity and high in stability, the preparation method is easy and fast to operate, and a result is easy to judge and read.

Description

technical field [0001] The invention relates to a rapid detection IgG subtype classification reagent card and a preparation method thereof. Background technique [0002] Immunoglobulin (IgG) is the main molecule of human immunity, accounting for 75% of the total immunoglobulin in the plasma of healthy people. When a certain pathogen invades the human body, its surface antigen can be used as a substance to stimulate the human immune system , so that the body can produce specific immunity to it, which comes from antibodies produced in the body. Since a decrease in the level of one IgG subtype can be accompanied by an increase in the level of one or several other IgG subtypes, the total IgG level remains normal. Therefore, even if the total amount of IgG is in the normal range or slightly lower than the normal range, it is necessary to measure the IgG subtype level. [0003] During the long pregnancy, due to the reality of fetal-maternal hemorrhage, a small amount of fetal bl...

AI Technical Summary

Problems solved by technology

The ELISA detection reagent of Sigma Company in the United States can detect the total IgG subclasses in the blood, but it cannot detect the blood group-specific IgG subclasses. It has limited reference value for clinical diagnosis and treatment, and the method takes a long time to check and is not suitable for single samples and emergency departments. instant detection

Images