Isoelectric focusing electrophoresis apparatus

A technology of isoelectric focusing electrophoresis and focusing tank, which is applied in the field of biotechnology separation and detection equipment, can solve the problems of unsuitable for large-scale experimental exploration, parameter value difference, low stability, etc., to avoid experimental errors and instability, The effect of reducing focusing batches and shortening focusing time

Inactive Publication Date: 2017-09-29
苏州伯楷安生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Multiple factors such as temperature, current, and electrophoretic medium will affect the test results. The parameter values ​​of various factors will have

Method used

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  • Isoelectric focusing electrophoresis apparatus
  • Isoelectric focusing electrophoresis apparatus
  • Isoelectric focusing electrophoresis apparatus

Examples

Experimental program
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Effect test

specific Embodiment example 2

[0024] combine figure 2 To describe this embodiment, figure 2 Focusing slots for bulk mode, including: positive electrode tip system, negative electrode tip system and bulk mode focusing slot. The focus tank adopts channel electrophoresis, and the whole block mode is a focus tank of whole block mode, which has 36 focusing channels, and can run 36 gel strips focus electrophoresis at the same time. When in use, place the focusing groove on the temperature control plate of the instrument. For the whole block mode, a focusing groove can be placed, and a maximum of 36 gel strips can be placed in the focusing electrophoresis at the same time. After installing the positive and negative electrode terminal systems respectively, add the corresponding reagent solution, set the required voltage and current, and plug in the power to start electrophoresis. After focusing, store the data in sequence, turn off the power and take out the focused strip for subsequent experiments.

specific Embodiment example 3

[0026] The difference between this embodiment and Example 1 is that two independent block mode focusing tanks 3 can be placed in the large-scale protein detection focusing tank device for isoelectric focusing electrophoresis, and 24 gels can be run at the same time. strip. Others are the same as in Example 1. This embodiment is used for two-dimensional electrophoresis, which improves the separation and purification efficiency and stability of two-dimensional electrophoresis substances.

specific Embodiment example 4

[0028] The difference between this embodiment and Example 1 is that one independent block-mode focusing tank 3 can be placed in the aforementioned large-scale protein detection focusing tank device for isoelectric focusing electrophoresis. Others are the same as in Example 1. This embodiment is used for two-dimensional electrophoresis for separation and purification of a small amount of protein or nucleic acid.

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Abstract

The invention discloses an isoelectric focusing electrophoresis apparatus, comprising a positive electrode-end system, a negative electrode-end system and a focusing tank. By means of a manner of operating a plurality of sub-block mode focusing tanks and a single whole-block focusing tank, the number of adhesive tapes operated in the electrophoresis apparatus reaches 13 to 36, thus greatly improving the operation efficiency; a great number of substances can be separated and purified in batches as few as possible, so that result errors brought by variation of multiple influence factors between multiple batches are reduced, and the stability is improved; meanwhile, the efficiency of separation and purification is improved, the operation period is shortened, the cost is reduced, and the input of manpower and material resources is saved.

Description

technical field [0001] The invention relates to the field of biotechnology separation and detection equipment, in particular to protein isoelectric focusing electrophoresis equipment. Background technique [0002] At present, protein research is a research hotspot in the post-gene era. In the study of protein separation and enrichment, two-dimensional electrophoresis (Two-dimensional electrophoresis, 2DE) is a very commonly used classic technique. In this technology, isoelectric focusing (IEF) electrophoresis is an important link, and using 7cm gel strip focusing electrophoresis for experimental exploration and medical sample detection is a relatively common and convenient method. [0003] At present, there are various isoelectric focusing electrophoresis instruments equipped with 7cm gel strip focusing grooves on the market, but at most 12 gel strips can be run at the same time at one time. For the detection of various samples, focusing experiments can only be performed mu...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/44795
Inventor 李国庆刘小平孔凡志
Owner 苏州伯楷安生物科技有限公司
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