A kind of single spore isolation method of Physiological Race of Plasmodium Chinese cabbage

Abstract

The invention relates to a single-spore isolation method for plasmodiophora brasicae. The method is characterized by including: freezing box filter paper culture: transferring an agar block containing single spore into a freezing box, putting the freezing box on a filter paper cultivated Chinese cabbage seedling root for inoculation; firstly conducting dark culture in the freezing box; letting the agar block contact root hairs to facilitate plasmodiophora infection; transition culture: conducting hydroponic culture after 6 days of growth, transferring the seedling into a centrifugal tube containing distilled water, putting the centrifugal tube into a light-tight centrifugal tube box and conducting culture in an illumination culture box in an environment with a temperature of 23DEG C, a humidity of 60% and illumination of 16h / d for 2 days, then using a Hongland's nutrient solution to conduct hydroponic culture, replenishing the nutrient solution every day until the root hair is infected, and then transferring the seedling into a matrix for culture; when the seedling grows to 4 true leaves, conducting sterile soil culture, and 40 days later, collecting plasmodiophora for physiological race identification. The method provided by the invention can increase the incidence rate to 47.86%, and enhances the plant survival rate from 27% to 67% through inoculation seedling period selection and culture condition optimization.

Description

technical field The invention relates to a method for isolating single spores of Plasmodium phylloxera of Chinese cabbage, is a technique for separating physiological races of plant pathogenic bacteria, and belongs to the field of biotechnology. Background technique Clubroot of cruciferous crops, also known as root cancer, is a worldwide soil-borne disease caused by the infection of Plasmodiophora brassicae, which seriously affects the production of Chinese cabbage and other cruciferous crops. Since Plasmodium usually infects in a mixed state of multiple physiological races, its resistance cannot last long. Therefore, it is of great application value to isolate various physiological races of Plasmodium through isolation technology of single spores of Plasmodium. At present, there have been some reports on the identification of physiological races using the single spore isolation technology of Pluzobacterium spp., for example, in 2008, Xue et al. used the method of culture i...

AI Technical Summary

Problems solved by technology

From these research results, it can be seen that the physiological races isolated by the existing technology are very limited. The main reason is that the separation technology is not perfect, especially the infection rate is too low and the seedling rate is not high.

Images