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Immunofluorescence detection card for determining chloramphenicol, and making method and application thereof

An immunofluorescence detection and immunofluorescence technology, which is applied in the field of immunofluorescence analysis, can solve problems such as insufficient sensitivity, and achieve the effect of high detection sensitivity and convenient use

Inactive Publication Date: 2017-10-13
深圳市三方圆生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the detection card and detection method for detecting chloramphenicol at present all have the problem of insufficient sensitivity, and it is necessary to provide a detection card and detection method with higher sensitivity

Method used

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  • Immunofluorescence detection card for determining chloramphenicol, and making method and application thereof
  • Immunofluorescence detection card for determining chloramphenicol, and making method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] refer to figure 1 , figure 1 It is a structural schematic diagram of the immunofluorescence detection card for determining chloramphenicol in Example 1. This embodiment provides an immunofluorescence detection card for determining chloramphenicol, including a backing plate 1, and also includes a backing plate 1 attached to the backing plate 1. And the sample pad 2, the coupling pad 3, the reaction membrane 4 and the water-absorbing pad 5 that are closely connected in sequence, the chloramphenicol antibody labeled with fluorescent microspheres on the coupling pad 3, and the reaction membrane 4 is equipped with a detection Line 6 and quality control line 7, the detection line 6 is close to the conjugate pad 3, the quality control line 7 is close to the water-absorbing pad 5, and the detection line 6 is coated with chloramphenicol antigen, so The quality control line 7 is coated with a secondary antibody that recognizes the chloramphenicol antibody.

[0033] The above-me...

Embodiment 2

[0041] The immunofluorescence detection card provided in this embodiment for determining chloramphenicol is basically the same as that in Example 1, except that the immunofluorescence detection card also includes an upper casing 9 and a lower casing 8, and the upper casing 9 is covered on Above the sample pad 2, the conjugate pad 3, the reaction membrane 4 and the water-absorbing pad 5, the lower housing 8 is arranged below the backing plate 1, the upper housing 9 and the The lower shells 8 are mutually covered, and the periphery of the lower shell 8 is provided with locking teeth 11, and the periphery of the upper shell 9 is provided with locking holes 10 matched with the locking teeth 11. The upper shell 9 and the lower shell 9 The housing 8 is connected to the locking hole 10 through the locking teeth 11 . The upper housing 9 is provided with a sample injection hole 12 at a position corresponding to the sample pad 2, and the sample to be determined is added from the sample ...

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Abstract

The invention discloses an immunofluorescence detection card for measuring chloramphenicol, and a making method and an application thereof. The detection card comprises a pad plate, and a sample pad, a conjugate pad, a reaction film and an absorbent pad which are attached to the pad plate and sequentially closely connected, the conjugate pad is provided with a fluorescence microsphere-labeled chloramphenicol antibody, the reaction film is provided with a detection line and a quality control line, the detection line is close to the conjugate pad, the quality control line is close to the absorbent pad, the detection line is coated with a chloramphenicol antigen, and the quality control line is coated with a secondary antibody for identifying the chloramphenicol antibody. The making method comprises the following steps: making the sample pad, making conjugate pad, making the reaction film, making the absorbent pad, and assembling above objects. A sample to be measured is added to the sample pad and undergoes an immunoreaction, the immunofluorescence detection card for measuring chloramphenicol is placed in a fluorescence card reader, and the fluorescence card reader reads the intensity of a fluorescence signal to obtain a detection result. The detection card for determining the chloramphenicol is fast and convenient to use, and has a high sensitivity reaching 0.05 ng / mL.

Description

technical field [0001] The invention relates to the technical field of immunofluorescence analysis, in particular to an immunofluorescence detection card for determining chloramphenicol and a preparation method and application thereof. Background technique [0002] Chloramphenicol (Chloramphenicol, CAP) is a widely used antibiotic, which once played an important role in the control and treatment of livestock and poultry diseases. However, chloramphenicol residues can cause human aplastic anemia, agranulocytosis and other diseases, and developed countries such as Europe and the United States have banned the use of them. Once the export product is found to exceed the standard, it will be prohibited from export. At present, the main method for the detection of chloramphenicol residues is instrumental analysis, which is not conducive to wide application because of the tedious and time-consuming sample pretreatment process and high detection cost. [0003] Immunofluorescence an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533G01N33/58
CPCG01N33/9446G01N33/533G01N33/558G01N33/582G01N33/585
Inventor 钟松清谭攀孙晶玮王军亮
Owner 深圳市三方圆生物科技股份有限公司
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