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Devices for high-throughput aggregation and manipulation of mammalian cells

A cell and channel technology, which can be used in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc., and can solve problems such as limitations

Pending Publication Date: 2017-10-17
ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] -) The flux is limited due to hole spacing (hole spacing);

Method used

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  • Devices for high-throughput aggregation and manipulation of mammalian cells
  • Devices for high-throughput aggregation and manipulation of mammalian cells
  • Devices for high-throughput aggregation and manipulation of mammalian cells

Examples

Experimental program
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Embodiment Construction

[0029] In one embodiment, the present invention relates to a device for collecting cells, said device comprising at least one cavity, wherein said cavity comprises a plurality of microwells for receiving at least one cell, wherein each said well comprises a vertical Side walls and curved bottom.

[0030] Preferably, the device comprises a plurality of cavities, each of said cavities comprising a plurality of microwells.

[0031] In one embodiment, the diameter (d), height (h) and distance between the pores (pitch, p) of the microwells are uncorrelated and can be varied independently of each other.

[0032] In one embodiment, the micropores have an opening diameter of 1 μm to 3 mm.

[0033]In one embodiment, the microwells have a height (h) of 1 μm to 3 mm.

[0034] In one embodiment, the microwells have cavities of different sizes or shapes.

[0035] In one embodiment, the spacing between the microwells (pitch size) is so small that cells that fall within the area of ​​the ...

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Abstract

The invention concerns a device (1) for aggregating cells comprising at least one cavity (2), wherein said cavity comprises a plurality of microwells (3) for receiving at least one cell, wherein each said well comprises a vertical sidewall and a curved bottom.

Description

technical field [0001] The present application relates to a device for high-throughput aggregation of cells and long-term culture of said cells and manipulation of said cells within said device. The cell aggregates are used in basic biology, in particular developmental and cancer biology, regenerative medicine and drug screening. [0002] The invention also relates to a method for manufacturing said device. Background technique [0003] Tissue self-organizes as a complex three-dimensional entity of specialized cells, adjacent supporting cells, extracellular matrix components, and other structural and dimensional elements that continuously crosstalk to ensure and maintain function (Li et al. 2005). This multicomponent microenvironment, known as the niche, has been minimally simplified in the classical two-dimensional cell culture system used as a standardized platform for basic research in the study of cell types of interest from research Developmental biology of differenti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCC12M23/12C12M23/16C12M23/20B01L2200/026B01L2200/0668B01L2300/0829B01L2300/0851B01L2300/0893B01L2300/165B01L3/5085C12M1/32C12M3/06
Inventor 西拉克·赫内尔纳塔莉·布兰登贝格尔马蒂亚斯·卢图福
Owner ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)