Apparatus for large-scale culture and freezing of hepatocytes

Abstract

The invention relates to a device for large-scale culture and freezing of hepatocytes. The device includes a cell culture component, a freeze component and a liquid nitrogen tank. The cell culture component is installed in the freeze component through a first moving component, and the cell culture component can be The assembly enters into the freezing assembly or moves out of the freezing assembly, the freezing assembly is installed in the liquid nitrogen tank through the second moving assembly, and the freezing assembly can enter into the liquid nitrogen tank or move out of the liquid nitrogen tank through the second moving assembly. The beneficial effects of the present invention are: the hepatocytes can be cultured through the culture box, the large-scale culture of the hepatocytes can be realized through the stacked supporting plates and the cell culture fiber membrane, and the freezing can be realized through the freezing box. and the second moving component can flexibly adjust the movement of the culture box and the freezing box, so that the large-scale cultivation of hepatocytes can be completed through the integrated equipment, thereby realizing the large-scale finished product output of hepatocytes.

Description

technical field [0001] The invention belongs to the technical field of hepatocyte culture, in particular to a device for large-scale culture and freezing of hepatocytes. Background technique [0002] Hepatocytes are the parenchymal cells of the liver and are the main cells that make up the liver, accounting for 80% of the volume and number of the liver. They are highly differentiated cells. Liver cells have active metabolism, which can synthesize essential coagulation factors, fatty acids, cholesterol, phospholipids, etc., and store glycogen, protein, lipids and vitamins for the needs of the body. Nowadays, patients with chronic hepatitis cirrhosis and advanced liver cancer often die of end-stage liver failure. The application of bioartificial liver and liver transplantation to treat liver failure has made remarkable achievements in recent years. The bioartificial liver support system removes various harmful substances in the body and supplements the necessary proteins and ...

AI Technical Summary

Problems solved by technology

The freezing of liver cells also requires additional freezing equipment for freezing. In the case of frequent operations and cumbersome processes, the method of culturing and freezing liver cells alone can no longer meet the growing demand for liver cells. A comprehensive integrated device capable of large-scale culture, freezing and recovery of hepatocytes for the cultivation of hepatocytes, so as to meet the large-scale finished product output of hepatocytes

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