Original ecological imitative wild cultivation method for inonotus sanghuang
A technology imitating wild cultivation and Phellinus japonica, applied in botany equipment and methods, horticulture, application, etc., can solve problems such as difficult large-scale cultivation, impact on the quality of Phellinus japonica, and harsh cultivation conditions, so as to reduce production costs and The effects of labor intensity, stable mulberry area, and simple cultivation methods
Examples
Embodiment 1
[0019] 1. Isolation of Phellinus bacterium and cultivation of Phellinus mother species
[0020] Phellinus fruiting bodies with large flowers, thick flesh, bright yellow gills, and no pests and diseases were selected from mulberry trees as the separation material. After rinsing and disinfecting the surface of Phellinus fruiting body, cut the fruiting body under aseptic conditions, pick about 0.5 cm square tissue pieces in the middle layer of the fruiting body and place them on the mother seed medium plate at a certain distance, each plate Six pieces were cultured at about 28°C. After 24 hours, fine and fine hairy hyphae grew on the tissue pieces. Use an inoculation knife to transfer the apex mycelium into the mother medium, and culture at about 25°C for 10 days. Mycelia covered the slope.
[0021] If mushy viscous colonies or fast-growing fungal colonies are found, the tubes will not be transferred and re-separation is required. Phellinus strains can also be separated by spo...
Embodiment 2
[0042] Concrete method is the same as embodiment 1, and difference is: The mother seed medium adopts the following formula: 50 g of mulberry branches, 20 g of fresh bran, 200 g of potatoes, 20 g of glucose, 18 g to 23 g of agar, 1.5 g of ammonium sulfate, 1 g of potassium dihydrogen phosphate, and 0.5 g of magnesium sulfate.
[0043] Preparation method of mother seed medium: Weigh the required raw materials according to the formula, add 500-600ml of water to mix the mulberry twig scraps and fresh bran to boil the juice, filter with gauze, and get the filtrate. Peel the potatoes, dig out the bud eyes, and slice. After the potato slices are boiled, filter them with gauze, add the filtrate to agar and glucose, heat until the agar dissolves, mix with the mulberry twigs and fresh bran filtrate prepared above, and make up to 1000ml of water to adjust the pH to 5-6.5. Divide into test tubes while hot. The medium capacity of each test tube accounts for about 1 / 5 of the height of t...
Embodiment 3
[0045] The specific method is the same as in Example 1, the difference is that the wooden nail culture medium has a formula by weight: 45% of mulberry branch, 20% of mulberry sawdust, 16.6% of bran, 16% of corn flour, 1.2% of sugar, carbonic acid Calcium 1.2%, plus ammonium sulfate 0.5%. The diameter of the mulberry branch is 0.7 centimeters, 4 centimeters long, and one end is pointed.
PUM
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Description
Claims
Application Information
- IPC
- A01G1/04
- Inventors
- 郑社会; 余建妹